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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (24): 96-101.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

5株氟罗沙星单克隆抗体杂交瘤细胞株的品质评价

谭成成1,刘艳霞2,李晓艳3,万忠海3   

  • 收稿日期:2009-07-06 修回日期:2009-09-02 出版日期:2009-12-20 发布日期:2009-12-20
  • 基金资助:

    军队卫生十一五面上项目(20061105043)资助

Quality Evaluation on the Monoclonal Antibodies of Against Fleroxacin of Five Hybridoma Cells

  • Received:2009-07-06 Revised:2009-09-02 Online:2009-12-20 Published:2009-12-20

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摘要：

摘 要：通过对5株杂交瘤细胞株分泌稳定性、抗体亚型、亲和力、特异性等方面的测定，表明其染色体众数为87-90条，具有众数的细胞所占比例为81-84%，单抗亚型均为IgG1型，腹水单抗的亲和常数为108～109M－1，氟罗沙星单抗与诺氟沙星、环丙沙星、恩诺沙星、链霉素、庆大霉素、卡那霉素的交叉反应试验表明。所制备的抗氟罗沙星单抗对氟喹诺酮类药物有较高的交叉反应率。与链霉素、庆大霉素、卡那霉素无交叉反应。

关键词: 溶血素, 溶血素, 穿孔毒素, 细胞溶素

Abstract:

Abstract:The quality of the prepared anti-Fleroxacin monoclonal antibodies were evaluated by the stability of secretion, antibody-subtype , affinity and specificity experiments’results. It showed that the five strains of hybridoma cells contained modal number of chromatin for 87-90 which occupied for 81-84%, subtypes of monoclonal antibodies were all IgG1 types, affinity constant of ascites monoclonal antibodys was 108－109M-1.The cross-reaction experiments of Fleroxacin monoclonal antibody were carried out with Norfloxacin,Ciprofloxacin,Enrofloxacin,Sreptomycin,Gentamicin,Kanamycin.From the experiments we know that the prepared anti-fleroxacin monoclonal antibodies had a very high reaction rate with fluoroquinolones, but had no reaction with magnamycin, kanamycin, streptomycin.