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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (24): 102-107.

所属专题： 生物技术； 食用菌； 食用菌

• 食品 营养 检测 安全 • 上一篇    下一篇

蔵灵菇源克鲁维酵母M3菌株胆盐水解酶的特性研究

刘 慧,何 欢,张雪娇,潘昌莉,张红星   

  • 收稿日期:2009-09-22 修回日期:2009-10-25 出版日期:2009-12-20 发布日期:2009-12-20
  • 基金资助:

    藏灵菇克鲁维酵母菌降胆固醇作用及机理的研究

Studies on the Characteristics of Bile Salt Hydrolase Produced by Kluyveromyces marxianus M3 from Tibetan Kefir

  • Received:2009-09-22 Revised:2009-10-25 Online:2009-12-20 Published:2009-12-20

摘要：

摘 要：探讨蔵灵菇马克斯克鲁维酵母M3菌株胆盐水解酶的特性及其发酵动力学原理。采用单因素多水平试验，在pH4~8、温度31~43℃、底物浓度4~8 mmol/L及不同化学试剂(SDS、EDTA、尿素、Cu2+ 、Mg2+、Ca2+、Fe3+、Al3+、Mn2+)的条件下，胆盐水解酶与底物反应30min，检测酶活力。胆盐水解酶最适反应条件：pH为6.0、底物浓度为7 mmol/L、温度为37℃，Mn2+、Fe3+、Ca2+金属离子对酶活性有较大提高作用，其他化学试剂对酶活性影响不大。胆盐水解酶的酶促反应动力学常数Km为2.60 mmol/L。M3菌株在18~21 h进入稳定期，18h对数生长期时酶活性达到最高，表明其胆盐水解酶发酵动力学类型为生长偶联合成型。

关键词: 彩叶植物, 彩叶植物, 花青苷, 酶, 分子机制

Abstract:

Abstract: To investigate the characteristics and dynamic principle of the bile salt hydrolase produced by Kluyveromyces marxianus M3 from Tibetan Kefir, the single factor and multi levels orthogonal experiments was applied. Enzyme activity was tested under the different contitions:pH4~8, temperature31~43℃, concentration of substrate 4~8mmol/L, and different chemical reagents(SDS、EDTA、carbamide、Cu2+、Mg2+、Ca2+、Fe3+、Al3+、Mn2+ )during 30 min reaction between bile salt hydrolase and substrate. The optimal reaction condition of bile salt hydrolase was pH6.0, concentration of substrate 7mmol/L, temperature37℃. Metal ion of Mn2+、Fe3+、Ca2+ had comparatively large elevation effect on the enzyme activity, and other chemical reagents had few effect on the enzyme activity. The kinetic constant Km of bile salt hydrolase was 2.60 mmol/L. Strain M3 entered the stationary phases after 18~21h. The enzyme activity reached the maxlmum during log phase growth at18h. The experiments results showed the category of dynamic principle was growth couple synthesis type.