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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (2): 68-70.

所属专题： 水稻

• 生物技术科学 • 上一篇    下一篇

适宜水稻花粉萌发的液体培养基的优化

温婵,罗海伟,彭富海,王生银,田小海   

  • 收稿日期:2009-08-12 修回日期:2009-12-07 出版日期:2010-01-20 发布日期:2010-01-20
  • 基金资助:

    长江大学国家大学生创新性实验计划项目

Screening for Ingredients of a Liquid Culture Medium for Rice Pollen Germination Test

  • Received:2009-08-12 Revised:2009-12-07 Online:2010-01-20 Published:2010-01-20

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摘要：

花粉萌发率是检测花粉活力的一个重要指标，但实践中还缺少便捷、可靠的水稻花粉萌发率测定方法。本研究在前人研究基础上，采用液体培养基对相关培养条件进行了优化。结果表明，采用155g/L蔗糖+20mg/L H3BO3+40mg/L CaCl2+5 mg/L VB1相配合的培养基较适合两系杂交中稻两优818花粉的萌发，萌发率最高可达63.78%。此外，该培养方法具有培养条件要求不高、发芽结果稳定性好、培养基分布均匀等优点。

关键词: 土地承包经营权, 土地承包经营权, 流转市场, 适度规模经营

Abstract:

The pollen germination rate (PGR) is one of the most important factors in evaluating the pollen vitality. Practically, low-cost, high-speed and feasible culture methods for determination of PGR are lacking. In the present study, we tried to screen the ingredients of a liquid culture medium for rice pollen germination test based on some previous studies. The results indicated that, with a two-line hybrid rice cultivar Liangyou 818, pollen germination rate in the culture medium with a ingredient of 155g/L sucrose +20mg/L H3BO3+40mg/L CaCl2+5 mg/L VB1 showed significantly greater than the other ingredient-based media, with a highest PGR 63.78%. In addition, this culture method was characterized by low-cost, stability in PGR, and evenly distribution of pollens in the surface of the culture medium.