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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (2): 64-67.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

微小毛霉（Mucor.pusillus）△6-脂肪酸脱饱和酶基因的克隆与表达

吴建勇 黄华磊   

  • 收稿日期:2009-08-24 修回日期:2009-09-29 出版日期:2010-01-20 发布日期:2010-01-20
  • 基金资助:

    国家高技术研究发展计划

Cloning and Expression of △6 -Fatty acid Desaturase Gene From Mucor.pusillusv

  • Received:2009-08-24 Revised:2009-09-29 Online:2010-01-20 Published:2010-01-20

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摘要：

目的：微小毛霉（Mucor.pusillus）△6-脂肪酸脱饱和酶基因的克隆与表达。方法：以一株产γ-亚麻酸的微小毛霉为材料，通过PCR方法克隆得到的△6-脂肪酸脱饱和酶基因，构建重组的酵母表达载体pYMpD6D，将重组载体通过醋酸锂方法导入酿酒酵母中，并利用酵母表达系统证实该基因能导致酵母合成γ-亚麻酸。结果：通过GC分析，γ-亚麻酸占总脂肪酸量的6.53％,微小毛霉△6-脂肪酸脱饱和酶基因在酵母中得到了正确的表达。本实验为进一步研究△6-脂肪酸脱饱和酶基因家族和进一步利用该基因来改良作物奠定了一定的基础。

关键词: 聚合草, 聚合草, 蛋白质, 氨基酸, 测定, 评价

Abstract:

Objective:Cloning and Expression of △6-Fatty acid Desaturase Gene From Mucor.pusillusv.Methods:The research used a strain of GLA produceing Mucor, through PCR method, got a △6-fatty acid desaturase gene of Mucor.pusillus,and generated a recombinant vector pYMpD6D.The recombinant plasmid pYMpD6D was transformed into S. cerevisiae INVScl using lithium acetate method, and using the yeast expresses system pYES2.0 to confirm that this gene can cause the yeast to synthesis GLA. Results:Though GC analyze, the GLA content about 6.53％ in total fatty acid.This research broaden a way for deep research on △6-fatty acid desaturase gene family,and lay certain foundation for further uses this gene to improve the crops quality.