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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (4): 300-303.

所属专题： 生物技术； 园艺

• 农学 农业基础科学 • 上一篇    下一篇

火龙果总DNA提取方法比较研究

余志雄1,欧高政1,陈清西2,袁亚芳1   

  • 收稿日期:2009-08-28 修回日期:2009-12-13 出版日期:2010-02-20 发布日期:2010-02-20
  • 基金资助:

    福建省农业科技重点项目

Study on Comparison of Methods for Pitaya Total DNA Extraction

  • Received:2009-08-28 Revised:2009-12-13 Online:2010-02-20 Published:2010-02-20

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摘要：

摘要：火龙果组织中含有多糖及其他次生代谢物质，因而难以提取高质量的DNA。本试验以火龙果嫩茎为材料，进行多种DNA提取方法比较研究，并通过琼脂糖凝胶电泳对所提取的DNA样品进行检测，结果表明，改良CTAB法和微量CTAB法均可用于火龙果DNA提取，尤其是微量CTAB法，提取的DNA纯度较为理想，能满足后续分子生物学研究的要求。

关键词: 烤烟, 烤烟, 品种, 产质量, 抗性

Abstract:

Abstract: The tissues of Pitaya richly contain polysaccharide and other subsequent metabolizing substance, so it is difficult to extract DNA effectively. The study compares different methods for Pitaya DNA extraction on tender stem of Pitaya.. Then the purified DNA was detected through Eppendorf Biophotomete. The researches indicated that modified CTAB and micro-CTAB extraction methods were both suitable for extracting DNA of Pitaya, especially the latter. DNA purity gained by those two methods was available to the further molecular biology study.