欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (4): 200-204.

• 林学 园艺 园林 • 上一篇    下一篇

自由人槭‘秋焰’（Acer×freemanii ‘Autumn Blaze’）组培的褐变控制研究

招雪晴1,苑兆和1,徐榕2,尹燕雷1,冯立娟1   

  • 收稿日期:2009-09-09 修回日期:2009-09-24 出版日期:2010-02-20 发布日期:2010-02-20

Studies on Browning Control in Tissue Culture of Acer×freemanii ‘Autumn Blaze’

  • Received:2009-09-09 Revised:2009-09-24 Online:2010-02-20 Published:2010-02-20

PDF (PC)

1090

摘要：

针对自由人槭‘秋焰’（Acer×freemanii ‘Autumn Blaze’）初代培养外植体褐化严重问题，采取不同取材时间、添加抗褐变剂、暗处理等措施，研究了三种措施对自由人槭‘秋焰’组织培养中褐化的影响效应。结果表明：随着取材月份变化，外植体褐化率提高；AC和PVP对褐变的处理，与Vc和Na2S2O3的处理相比达到差异极显著，又以2.0g/L的PVP抑制褐化效果最佳；随着黑暗处理时间的加长，褐化率呈现高-低-高的变化趋势。春季4、5月份取材，接种于添加PVP 2.0g/L的MS上时，直接进行光照培养，可有效控制褐变。

关键词: 大蒜, 大蒜, Nacl胁迫, pH胁迫, 细胞分裂, 大蒜, Nacl胁迫, pH胁迫, 细胞分裂

Abstract:

The measures including different collecting time, adding antibrowning reagents and culturing in darkness were carried out in terms of severe browning phenomenon in the process of Acer×freemanii ‘Autumn Blaze’ tissue culture. The effects of three browning control measures were studied in the paper. The results were as follows: The browning rate increased with months changed; The antibrowning effects with AC and PVP showed extremely significant difference with contrast to that of Vc and Na2S2O3. The browning control effects of PVP with concentration of 2.0g/L was optimal; The browning rate presented higt-low-high tendency with the darkness time increased. Measures of collecting explants in April and May, inoculating on MS adding 2.0 g/L PVP, culturing under the light directly could control browning effectively.