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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (6): 31-34.

所属专题： 小麦

• 生物技术科学 • 上一篇    下一篇

黄淮麦区部分小麦品种（系）1Dx5+1Dy10的分子检测与分析

王静,刘东涛,冯国华,赵军海,陈荣振   

  • 收稿日期:2009-10-27 修回日期:2009-11-23 出版日期:2010-03-20 发布日期:2010-03-20
  • 基金资助:

    江苏省农科院研究基金项目

Molecular Detection and Analysis of 1Dx5+1Dy10 in Partial Wheat Cultivars(Lines) From Huang-Huai Wheat Region of China

  • Received:2009-10-27 Revised:2009-11-23 Online:2010-03-20 Published:2010-03-20

PDF (PC)

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摘要：

目前在已报道的检测高分子量麦谷蛋白亚基1Dx5的PCR分子标记中，由引物Dx-F，Dx5-F和Dx-R组成的共显性标记，能稳定地扩增出5亚基和非5亚基的特异序列，并且无非特异PCR产物，是用于高分子量麦谷蛋白亚基1Dx5分子检测的最理想标记。本次实验利用此对引物, 通过PCR技术对221份小麦亲本进行了1Dx5亚基检测。结果表明，在所检测的材料中有35份材料携带1Dx5亚基，占所测材料总数的16.3%，远低于国外的基因频率（约85%）。

关键词: Bar基因, Bar基因, 转基因玉米, 基因漂移

Abstract:

At present, reported at detection of HMW-GS 1Dx5 molecular markers, the marker,composed of three primers Dx-F, Dx5-F and Dx-R, is a specific co-dominant PCR markers for 1Dx5 subunit,and has no non-specific amplicon.It was strongly recommended to be used in wheat molecular assiatant selection (MAS) breeding for good bread-marking quality. The experimental use of this pair of primers by PCR technology for 221copies of a parental wheat gene 1Dx5 subunit. The results showed that the detection of 35 copies of Materials Materials 1Dx5subunit carried, representing the total number of measured Materials16.3%, much lower than the frequency of foreign genes (about 85%).