欢迎访问《中国农学通报》,

中国农学通报 ›› 2014, Vol. 30 ›› Issue (30): 71-74.doi: 10.11924/j.issn.1000-6850.2014-1284

所属专题: 生物技术 玉米

• 农学 农业基础科学 • 上一篇    下一篇

利用叶蝉评价转基因玉米对非靶标生物的风险

冯艳杰,葛 阳,谭树乾,陈红星,郭艳艳,张恺祺,石旺鹏   

  1. 中国农业大学农学与生物技术学院农业昆虫与害虫防治,,,,,,
  • 收稿日期:2014-05-04 修回日期:2014-05-04 接受日期:2014-08-06 出版日期:2014-10-31 发布日期:2014-10-31
  • 通讯作者: 冯艳杰
  • 基金资助:
    国家转基因重大专项“转基因作物对非靶标节肢动物群落的影响”(2014ZX08011-003)

An Evaluation of Methods for Assessing the Impacts of Bt-maize on Non-target Organisms with Leafhoppers

  • Received:2014-05-04 Revised:2014-05-04 Accepted:2014-08-06 Online:2014-10-31 Published:2014-10-31

摘要: 转基因抗虫作物应用是替代杀虫剂的重要途径。但转基因作物可能存负面效应,特别是对非靶标生物的影响及害虫产生抗性。采用直接和陷阱调查结合方法,连续多年对转Cry1Ac 抗虫基因玉米实验田叶蝉和主要节肢动物数量动态跟踪调查,结果发现叶蝉是玉米田优势非靶标生物种群之一,中期发生比例占刺吸式害虫的28.9%~43.1%,且种群动态与主要非靶标节肢动物群落动态基本一致。又鉴于叶蝉直接刺吸接触到Bt 杀虫蛋白,可直接反映转基因作物对非靶标生物的安全性,因此可利用叶蝉作为转基因抗虫玉米安全性评价指示生物来评价转基因玉米对非靶标生物群落风险,田间测试结果证明此方法是可行的。

关键词: 淀粉, 淀粉

Abstract: The application of transgenic insect- resistant crops has been an important way to replace insecticides. But transgenic insect-resistant crops have potential negative effects, especially the effect on the non-target organisms and resistance to GM. Visual assessment and yellow traps were used for investigating the dynamic of leafhoppers and main arthropods in Cry1Ac transgenic insect-resistant corn field for two years. The study showed that planthoppers and leafhoppers were highly abundant and diverse in maize fields, their midterm occurrence was 28.9%-43.1% of sucking pest insects, and the population dynamic had no significant difference between Bt-maize and the untreated near-isogenic maize. Leafhoppers were directly exposed to these entomotoxic proteins and could also serve as a source of Cry protein exposure to predatory arthropods. Therefore, it was reasonable to use Auchenorrhyncha in the risk assessment of GM corn. So the risk of Bt corn on non-target organisms could be evaluated using leafhoppers as an indicator species. The results in field tests showed that this method was feasible.