欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (12): 98-105.doi: 10.11924/j.issn.1000-6850.casb2020-0247

所属专题： 植物保护； 玉米； 烟草种植与生产

• 植物保护·农药 • 上一篇    下一篇

烟草内生细菌YN2014042对烟草、玉米的定殖和促生作用研究

杨珍福1,2(), 何鹏飞1, 吴毅歆1, 何鹏搏1, 孔宝华1, 赵崇钧3, 刘剑金4(), 何月秋1()   

  1. 1云南农业大学,昆明 650201
    2中华全国供销合作总社昆明食用菌研究所,昆明 650221
    3微生物菌种筛选与应用国家地方发酵工程研究中心,昆明 650217
    4云南省烟草公司普洱市公司,云南普洱 665000
  • 收稿日期:2020-07-10 修回日期:2020-09-28 出版日期:2021-04-25 发布日期:2021-05-13
  • 通讯作者: 刘剑金,何月秋
  • 作者简介:杨珍福,女,1988年生,云南剑川人,助理研究员,硕士,主要从事植物病理学和菌种与资源研究。通信地址：650221 云南省昆明市五华区政教路14号 中华全国供销合作总社昆明食用菌研究所。E-mail： ynjcyzf152@126.com。
  • 基金资助:
    中国烟草总公司云南省公司项目“普洱市烤烟“两黑病”综合防控技术研究与应用”(2015YN26)

Endophytic Bacteria YN2014042: Colonization and Growth Promoting Effect on Tobacco and Maize

Yang Zhenfu1,2(), He Pengfei1, Wu Yiyin1, He Pengbo1, Kong Baohua1, Zhao Chongjun3, Liu Jianjin4(), He Yueqiu1()   

  1. 1Yunnan Agricultural University, Kunming 650201
    2Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperatives, Kunming 650221
    3National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650217
    4Pu'er Branch of Yunnan Tobacco Company, Pu’er Yunnan 665000
  • Received:2020-07-10 Revised:2020-09-28 Online:2021-04-25 Published:2021-05-13
  • Contact: Liu Jianjin,He Yueqiu

RichHTML

7

PDF (PC)

69

摘要：

为了开发利用烟草内生解淀粉芽孢杆菌YN2014042(YN42),对其在植物上的定殖和促生作用进行了研究。用自然转化法将携带绿色荧光蛋白基因的质粒pHAPII成功导入YN42细胞中,获得质粒稳定遗传和对烟草黑胫病菌、玉米穗腐病禾谷镰刀菌等13种病原真菌的拮抗能力与野生型YN42相同的GFP标记菌株YN42-GFP。用1.00×107 CFU/g的YN42-GFP分别浇灌烟草和玉米幼苗,在第50天时烟草根、茎和叶组织中仍回收到1.89×106 CFU/g、1.07×104 CFU/g和0.93×103 CFU/g的菌落;到第25天时玉米叶片中可回收到6.67×103 CFU/g的菌落;说明YN42在来源寄主和非来源寄主植物中都能稳定地定殖。将YN42添加到烟草育苗基质中制成微生物功能基质,与普通基质对比,1.00×107 CFU/g的菌株基质上的45天烟苗型高、地上部鲜重分别增长450.58%、756.52%;对玉米灌根30天处理,YN42处理的玉米苗型高和地上部鲜重分别增加19.37%和61.42%。研究结果表明YN42对多种病原菌有抑制作用,能在烟草和玉米上稳定定殖,对烟草和玉米的促生作用显著,在农业上具有良好的应用前景。

关键词: 烟草, 玉米, 内生细菌, 解淀粉芽孢杆菌, 绿色荧光蛋白标记, 拮抗作用, 定殖, 育苗基质

Abstract:

To utilize endophytic Bacillus amyloliquefaciens YN2014042 (YN42) isolated from tobacco, the colonization and growth promotion of YN42 were studied. We successfully obtained a stable GFP tagged strain YN42-GFP by using natural transformation with pHAPII plasmid introduced into YN42. Inhibition activity showed that the strain YN42-GFP exhibited the comparable ability as the wild type strain YN42 to inhibit13 pathogenic fungi such as Phytophthora parasitica var. nicotianae and Fusarium graminearum. When YN42-GFP was irrigated to tobacco and maize seedlings with 1.00×10 7 CFU/g, its colony number was respectively 1.89 × 10 6 CFU/g, 1.07×104 CFU/g and 0.93×103 CFU/g of tobacco root, stem and leaf tissues on the 50th day, and the colony number was 6.67×103CFU/g of maize leaf on the 25th day after inoculation, indicating that YN42 could colonize stably in both its indigenous host and non-host plants. When wild type of YN42 was added to tobacco seedling substrate with 1.00×107 CFU/g, the plant type height and fresh weight of aerial parts of 45-day-old tobacco seedling increased by 450.58% and 756.52%, respectively. When maize was drenched with 1.00×107 CFU/g, the plant type height and fresh weight of aerial parts of 30-day-old maize seedling increased by 19.37% and 61.42%, respectively. In conclusion, YN42 not only have a significant inhibitory effect on a variety of plant pathogens but also could colonize well in tobacco and maize, and promote the growth of tobacco and maize. YN42 has a good application prospect in agricultural production.

Key words: tobacco, maize, endophytic bacteria, Bacillus amyloliquefaciens, green fluorescence protein, antagonistic effect, colonization, seedling substrate

中图分类号: