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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (6): 47-50.

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

番茄子叶和下胚轴离体再生体系建立

梁美霞   

  • 收稿日期:2009-11-12 修回日期:2009-12-02 出版日期:2010-03-20 发布日期:2010-03-20

Establishment of regeneration system of cotyledon and hypocotyl in vitro of tomato

  • Received:2009-11-12 Revised:2009-12-02 Online:2010-03-20 Published:2010-03-20

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摘要：

以番茄无菌苗子叶和下胚轴为外植体，研究其在MS附加不同浓度6－BA和NAA的培养基上的分化情况。结果表明：在不同培养基上，番茄子叶和下胚轴均能诱导出淡绿色的愈伤组织和分化形成不定芽，但在不同的培养基上，番茄子叶和下胚轴的出愈率和不定芽诱导存在明显差别。诱导番茄愈伤组织形成和芽分化的最佳培养基分别为：MS+6-BA 2 mg/L+IAA 0.2 mg/L和MS+6-BA 2 mg/L+IAA 0.02 mg/L，番茄不定芽生根培养基为：MS+IAA0.2 mg/L。

关键词: 拟青山海关杨, 拟青山海关杨, 再生系统, 遗传转化, GUS活性

Abstract:

Abstract: The cotyledon and hypocotyl from tomato asepsis seedlings were cultured on the medium with different 6-BA and NAA concentrations to study their effects on the callus induction and shoot differentiation. The result indicated that some light green callus could be induced and adventitious buds could be differentiated in all kinds of medium, but there were obvious different in different medium. The optimum medium for tomato callus formation and bud differentiation respectively were MS+6-BA2 mg/L+IAA0.2 mg/L and MS+6-BA2 mg/L+I AA 0.02mg/L. The best medium for inducing tomato root was MS + IAA 0.2 mg/L.