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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (6): 51-53.

所属专题： 生物技术； 烟草种植与生产

• 生物技术科学 • 上一篇    下一篇

烤烟薄细胞层培养一次成苗与快繁研究

蒲文宣,杨亿军,孙在军,张新要,宋卫武,黄平俊   

  • 收稿日期:2009-09-14 修回日期:2009-12-16 出版日期:2010-03-20 发布日期:2010-03-20

One-step shoot Formation and Micropropagation in the thin cell-layer Cultures of Flue-cured Tobacco

  • Received:2009-09-14 Revised:2009-12-16 Online:2010-03-20 Published:2010-03-20

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摘要：

烤烟（Nicotiana tabacum L）嫩茎3～15层表皮和亚表皮薄细胞层在附加有适当激素配比的MS培养基上可一次培养成苗。薄细胞层一次培养成苗的适宜培养基为：MS+NAA0.1～0.3 mg/l +IBA0.1 mg/l +6-BA1.5～2.5 mg/l+3%蔗糖；BA浓度控制在2.5mg/l以下有利于培育壮苗。适宜的生根培养基为：1/2MS+2%蔗糖+NAA0.1～0.2 mg/l。

关键词: 肉仔鸡, 肉仔鸡, 大豆磷脂, 大豆活性肽, 生产性能, 胴体品质

Abstract:

The epidermal and sub- epidermal thin cell-layer of flue-cured tobacco (Nicotiana tabacum L) tender stem in additional appropriate hormone combinations of the MS culture medium can take one-step shoot formation. MS+NAA0.1～0.3 mg/l +IBA0.1 mg/l +6-BA1.5～2.5 mg/l+3%Sucrose culture medium is suitable for one-step shoot formation in thin cell layer cultures. BA concentrations below 2.5mg / l is conducive to nurturing strong seedling. 1/2MS+2% Sucrose +NAA0.1～0.2 mg/l culture medium is suitable for root induction.