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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (9): 34-41.

所属专题： 生物技术； 玉米

• 生物技术科学 • 上一篇    下一篇

对生叶序玉米与互生叶序玉米细胞分裂素调控基因差异分析

马庆,程郢,项艳,朱苏文,程备久   

  • 收稿日期:2009-11-26 修回日期:2010-01-14 出版日期:2010-05-05 发布日期:2010-05-05
  • 基金资助:

    转基因技术新方法研究;玉米额外随体的遗传及其与对生性状遗传变异关系的研究

Analysis of Differences between Decussate Maize and Alternative Maize in Cytokins Controling Gene

Ma Qing, Cheng Ying, Xiang Yan, Zhu Suwen, Cheng BeiJiu   

  • Received:2009-11-26 Revised:2010-01-14 Online:2010-05-05 Published:2010-05-05

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摘要：

摘要：本研究在明晰对生叶序玉米生长点的三大类内源细胞分裂含量不仅显著高于互生叶序玉米，而且随互生到对生到螺旋畸形叶序逐渐增大，以及CTK/IAA值是控制玉米叶序分化类型关键因素的基础上，搜索玉米细胞分裂素调控基因ZmRR1，ZmRR2，ZmRR3，ZmRR4， ZmRR5，ZmRR6，ZmRR7，ZmRR8，ZmRR9，ZmRR10的基因序列及其CDS序列，设计特异性上下游引物，通过对对生玉米及其近等基因系的互生玉米基因组进行PCR扩增、测序，证实了对生玉米、互生玉米在ZmRR1，ZmRR2，ZmRR3，ZmRR4， ZmRR5，ZmRR6， ZmRR7，ZmRR8，ZmRR9，ZmRR10基因序列结构上未见差异。RT-PCR分析结果证实ZmRR1，ZmRR2是根特异性表达基因，ZmRR3在根和叶中不表达，而ZmRR6在茎尖中不表达，对生玉米与互生玉米在不同时期、不同发育组织中其ZmRR1，ZmRR2，ZmRR3，ZmRR4， ZmRR5，ZmRR6 ，ZmRR7，ZmRR8，ZmRR9，ZmRR10基因的表达均未见差异，表明本实验室的对生叶序玉米突变体的形成机制与冷泉港实验室关于ZmRR3基因的Mu转座子插入突变导致对生叶序玉米突变体形成的机制不同。

关键词: 抗虫棉, 抗虫棉, 休眠, 发芽率, 新鲜不发芽种子比率

Abstract:

Abstract:Based on the Cytokinin levels and CTK/IAA in opposite maize were significantly higher than those of alternative maize and increased from alternative to opposite, to spiral. The sequence of ZmRR1，ZmRR2，ZmRR3，ZmRR4， ZmRR5，ZmRR6， ZmRR7，ZmRR8，ZmRR9，ZmRR10 genes and the CDS of them were found in GenBank according the report about opposite maize of cold spring label,, we analyzed genes and expression of them relative to the formation of different phyllotaxy. ZmRR1，ZmRR2，ZmRR3，ZmRR4， ZmRR5，ZmRR6， ZmRR7，ZmRR8，ZmRR9，ZmRR10 genes genes were amplified from genomic DNA of opposite and alterative plants. The results suggest that the ZmRR1，ZmRR2，ZmRR3，ZmRR4， ZmRR5，ZmRR6， ZmRR7，ZmRR8，ZmRR9，ZmRR10 genes do not change in opposite and alternative phyllotaxy maize. In different tissues including embrys ,sprouts,mature leaves,roots,and immature spikes, ZmRR1，ZmRR2 only expressd in root; ZmRR3 did not expressed in roots and leaves ; ZmRR6 did not expressed in sprouts; ZmRR4， ZmRR5， ZmRR7，ZmRR8，ZmRR9，ZmRR10 expressed in different tissues including embrys ,sprouts,mature leaves,roots and immature spikes. The transcriptional level of ZmRR1，ZmRR2，ZmRR3，ZmRR4， ZmRR5，ZmRR6， ZmRR7，ZmRR8，ZmRR9，ZmRR10 in the different tissues including embrys ,sprouts,mature leaves,roots,immature spikes present no difference between the two phenotypes of mazie with decussate phyllotaxy and alternative phyllotaxy. These results indicate the phyllotaxy formation of opposite and alterative phyllotaxy maize used in this study is not similar to the transport of Mu7 to ZmRR3 gene.