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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (9): 26-33.

所属专题： 小麦

• 生物技术科学 • 上一篇    下一篇

青藏高原老芒麦种质遗传多样性的SSR分析

鄢家俊1,白史且1,常丹2,游明鸿1,张昌兵1,李达旭1   

  • 收稿日期:2009-12-03 修回日期:2009-12-17 出版日期:2010-05-05 发布日期:2010-05-05
  • 基金资助:

    国家公益性农业行业专项;现代农业产业技术体系建设专项资金;国家“十一五”科技支撑计划项目

Genetic diversity of wild Elymus sibiricus L. germplasm from Qinghai–Tibetan Plateau in China detected by SSR markers

Yan Jiajun1, Bai Shiqie1, Chang Dan2, You Minghong1, Zhang Changbing1, Li Daxu1   

  • Received:2009-12-03 Revised:2009-12-17 Online:2010-05-05 Published:2010-05-05

PDF (PC)

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摘要：

利用SSR标记技术对来自青藏高原的52份老芒麦（Elymus sibiricus L）材料的遗传变异及亲缘关系进行了研究。18对SSR引物共扩增出236条清晰的条带，其中多态性条带204条，多态性位点率（PPB）为86.44%，每对引物扩增出7~20条带纹，平均为13.1条，多态性信息（PIC）含量为0.267~0.471之间，平均为0.35，SSR标记效率（MI）为3.98；材料间的遗传相似系数（GS）为0.622到0.895之间，平均GS值为0.766，52份种质的Nei’s 遗传多样性指数（He）为0.3286，Shannon指数（Ho）为0.4851，表明供试材料之间差异明显，具有较为丰富的遗传多样性。根据研究结果进行聚类分析，可将52份老芒麦材料分成5大类，具有相同地理来源或相似生境的材料趋向于聚为一类。

关键词: 黄瓜, 黄瓜, 1-MCP处理, 冷害, 贮藏

Abstract:

The genetic diversity of 52 E. sibiricus accessions were evaluated by SSR markers. A total of 318 fragments were identified with 18 SSR primers sets, of which 204 (86.48% ) were polymorphic, and each primer generated 13.1 fragments, the polymorphism information content (PIC) per primer was 0.267~0.471, with an average of 0.35, and the marker index (MI) of SSR was 3.98. The genetic similarity (GS) coefficient of the tested accessions ranged from 0.622 to 0.895, with an average of 0.766. The Nei’s index of diversity (He) of the 52 accessions was 0.3286, and the Shannon’s index (Ho) was 0.4851. These results suggested that there was rich genetic diversity among the tested wild resources of E. sibiricus. 52 accessions could be divided into five main groups by cluster and principal component analysis, the accessions from the same region or with the same habitat type tends to were classified into the same group, indicating the geographical distribution of genetic diversity of E. sibiricus.