欢迎访问《中国农学通报》,

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 226-229.

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

生物技术在柑橘砧木上的应用

方治军,杨义伶,黄春辉,辜青青,徐小彪   

  • 收稿日期:2009-12-14 修回日期:2010-01-05 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:

    江西省重大战略产品科技专项

Applications of Biotechnology on Citrus Rootstocks

Fang Zhijun, Yang Yiling, Huang Chunhui, Gu Qingqing, Xu Xiaobiao   

  • Received:2009-12-14 Revised:2010-01-05 Online:2010-05-20 Published:2010-05-20

摘要:

本文概述了当前国内外柑橘砧木生物技术尤其是基因工程技术和体细胞杂交技术在柑橘砧木种质改良和品种选育的研究现状与进展,可为我国柑橘砧木资源的研究与开发利用提供参考。基因工程在柑橘育种中的应用起步较晚,但近年来发展迅速,通过运用农杆菌介导法、附体腋芽转化-离体扩繁鉴定法、DNA直接导入法等基因工程技术对培育新型抗性柑橘砧木品种取得了一定的成果;在体细胞杂种的运用方面,通过原生质体融合,获得了大量具优良性状的杂种体细胞,并建立了多个抗病杂交后代群。目前,柑橘砧木生物技术的研究方兴未艾,现代生物技术在柑橘砧木资源多样性研究与种质改良方面仍是今后的主攻方向。

关键词: 甜樱桃, 甜樱桃, 实生繁殖, 变异系数

Abstract:

The research progresses of germplasms improvement and cultivars selection from biotechnology especially the genetic engineering and somatic cell hybridization in Citrus rootstocks at home and abroad at present were summarized in order to provide a solid basis for further study and development of Citrus rootstocks. Though genetic engineering started a little late, it gained a great development nowadays. Achievements has been gotten in raising novel Citrus rootstocks by genetic engineering methods such as Agrobacterium-mediated transformation,the method of possessed axillary bud transformation-identification of in vitro propagation, direction introduction of alien DNA and so on. For the use of somatic cell hybridization, a great number of hybridized cells were gotten, and many disease-resistant hybrid progeny groups were built by protoplast fusion. At the present time, the researches of biotechnology in Citrus rootstocks are just on the beginning, studies will be focused on the use of biotechnology in the resource diversity and germplasm improvement of Citrus rootstocks.