关键词: 实时荧光定量PCR, 实时荧光定量PCR, Ct值, 植物病害, 引物设计, 灵敏度

Abstract:

In order to search the best effects of plantation fertilizing treatment of Populus tomentosa Carr,the experimental studied the effects of plantation growth and nutritional status with different fertilizing time and regime by the orthogonal experiment design L9（34）.By measuring the changes of its growth indicators (diameter height ,crown width and a number of branches) and the nutrient content in leaves. The results showed that N was Mauritian. the effects of fertilization were in the order of N > K> P in the growth period, and the best N∶P2O5∶K2O fertilizing treatment recipe was 3:1.5:1.Diameter,height and crown width concentrated in June to 34.23%, 31.74% and 36.39% of total growing season .Fertilization treatments speeded up the diameter, tree height, crown width growth mainly concentrating in July .Compared with the control, the diameter, tree height, crown width growth was 147.06%, 23.26%, 45.71%.Fertilizer should be elected during the fast-growing pre-fertilization that is prior to June .If top-dressing, we should choose the period July .Fertilization affected the change of nitrogen, phosphorus and potassium in leafs , where the changes of nitrogen was obvious than the phosphorus and potassium.The promotion absorption of nitrogen, phosphorus, and potassium were the best deal with Treatment 7 (N 240g / strains, P2O5 0g / strain, K2O 80g / strain), treatment 6 (N 120g / strains, P2O5 120g / strains, K2O 0g / strain,,) and treatment 5 (N 120g / strains, P2O5 60g / plant, K2O 80g / strain).Therefore, choosing a reasonable fertilization ratio and a reasonable period of fertilization can significantly increase the growth of Populus tomentosa plantations.