2个MADS-box 基因在山葡萄雌花和雄花中的表达分析

doi:10.11924/j.issn.1000-6850.casb16040094

中国农学通报 ›› 2016, Vol. 32 ›› Issue (22): 122-126.doi: 10.11924/j.issn.1000-6850.casb16040094

所属专题：生物技术；园艺

2个MADS-box 基因在山葡萄雌花和雄花中的表达分析

沙玉芬¹,郑秋玲¹,张超杰¹,范书亭²,唐美玲¹

(¹山东省烟台市农业科学研究院,山东烟台 265500；²中国农业科学院特产研究所,吉林左家 132019）

收稿日期:2016-04-15 修回日期:2016-05-23 接受日期:2016-05-23 出版日期:2016-08-09 发布日期:2016-08-09
通讯作者: 唐美玲＊
基金资助:
山东省酿酒葡萄良种工程实验室创新能力专项；现代农业产业技术体系专项资金(nycytx-30)。

Two MADS-box Genes Expression Analysis in Male and Female Floral Development of Vitis amurensis Rupr.

Sha Yufen¹, Zheng Qiuling¹, Zhang Chaojie¹, Fan Shuting², Tang Meiling¹

(¹Yantai Academy of Agricultural Sciences, Yantai Shandong 265500; ²The Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Zuojia Jilin 132019)

Received:2016-04-15 Revised:2016-05-23 Accepted:2016-05-23 Online:2016-08-09 Published:2016-08-09

摘要/Abstract

摘要： 为了探明MADS-box基因在山葡萄性别分化中的作用，笔者利用实时荧光定量PCR技术对VvMADS5和VvMADS8 2个基因在山葡萄雌、雄花发育过程的表达情况进行分析。结果表明，2个基因在山葡萄雌花和雄花中都有表达，VvMADS8在雌花和雄花中的表达趋势以及表达量基本相同。VvMADS5在雌花和雄花中的表达类型相同，而其在雌花中的表达量显著高于雄花。VvMADS8在花芽膨大期（4月30日）表达量最高，而VvMADS5在花序展露期（5月14日）表达量最高。VvMADS8、VvMADS5参与山葡萄性别分化，但不是控制性别分化的关键基因。

关键词: 降雨量, 降雨量, 烤烟, δ¹³C值, 生理指标

Abstract: The paper aims to investigate the role of two MADS-box genes in controlling sex differentiation of Vitis amurensis Rupr.. Real-time PCR was performed to observe the expression profiling of VvMADS5 and VvMADS8 in male and female floral development of Vitis amurensis Rupr. The results indicated that: the two genes were expressed not only in the male floral but also in the female floral; the expression pattern and expression value in the male floral were almost the same as that in the female floral; the expression pattern of VvMADS5 between male and the female was the same, but the expression value in the female was significantly higher than that in the male floral; the expression of VvMADS8 appeared one peak value at bud intumescence stage (April 30), while the highest expression quantity of VvMADS5 appeared at inflorescence show stage (May 14). VvMADS5 and VvMADS8 participated in sex differentiation of Vitis amurensis Rupr., but were not the key genes of controlling sex differentiation.

沙玉芬,郑秋玲,张超杰,范书亭,唐美玲. 2个MADS-box 基因在山葡萄雌花和雄花中的表达分析[J]. 中国农学通报, 2016, 32(22): 122-126.

Sha Yufen,Zheng Qiuling,Zhang Chaojie,Fan Shuting and Tang Meiling. Two MADS-box Genes Expression Analysis in Male and Female Floral Development of Vitis amurensis Rupr.[J]. Chinese Agricultural Science Bulletin, 2016, 32(22): 122-126.

参考文献

[1] 董庆龙,冀志蕊,迟福梅,等.苹果MADS-box转录因子的生物信息学及其在不同组织中的表达[J].中国农业科学,2014,47(6):1151-1161.
[2] 林苗苗,赵长竹,顾红,等.甜樱桃B类MADS-box基因的分离与序列分析[J].果树学报,2011,28(6):1082-1085.
[3] 吕山花,孟征.MADS-box基因家族基因重复及其功能的多样性[J].植物学通报,2007,24(1):60-70.
[4] 吕晋慧,张启翔.花发育调控基因的应用研究进展[J].北方园艺,2004(6):17-18.
[5] Hardenack S, Ye D, Saedler H. Comparison of MADS box gene expression in developing male and female flowers of the dioecious plant white campion[J].Plant Cell,1994,6(12):1775-1787.
[6] Losa A, Caporali E, Spada. AOM3 and AOM4: MADS box genes expressed in reproductive structures of Asparagus afficialis[J].Sex plant Reproduction,2004,16:215-221.
[7] Ainsworth C, Crossley S, Buchanan-Wollaston V, et al. Male and female flowers of the dioecious plant sorrel show different patterns of MADS box gene expression[J].Plant Cell,1995,7(10):1583-1598
[8] Pfent C, Pobursky K J, Sather D N, et al. Characterization of SpAPETALA3 and SpPISTILLATA, B class floral identity genes in Spinacia oleracea ,and their relationsip to dexual dimorphism[J].Dev Genes Evol,2005,215(3):132-142.
[9] 张微,李锡香.黄瓜花芽性别分化的研究进展与展望[J].华北农学报,2015,30(增刊):74-80.
[10] 周琳璘,宋国琦,李红燕,等.一个与小麦雄性不育育性转换相关的MADS-box转录因子基因[J].作物学报,2008,34(4):598-604.
[11] 宗成文,房经贵,陶建敏.葡萄MADS-box家族基因保守片段的克隆与序列分析[J].果树学报,2007,25(1):27-32.
[12] Jér?me Grimplet José Miguel Martínez-Zapater, María José Carmona. Structural and functional annotation of the MADS-box transcription factor family in Grapevine[J].Grimplet et al. BMC Genomics,2016(17):80-113.
[13] Lekha Sreekantan, Mark R Thomas. VvFT and VvMADS8, the grapevine homologues of the ?oral integrators FT and SOC1, have unique expression patterns in grapevine and hasten flowering in Arabidopsis[J].Functional Plant Biology,2006,33:1129-1139.
[14] Paul K Boss, Elisabetta Sensi, Chi Hua, et al. Thomas. Cloning and characterization of grapevine (Vitis vinifera L.) MADS-box genes expressed during in?orescence and berry development[J].Plant Science,2002,162:887-895.
[15] 王壮伟,渠慎春,章镇.苹果属RNA高效快速提取新方法[J].果树学报,2004,21(4):385-387.
[16] Karen E Reid, Niclas Olsson, James Schlosser, et al. An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development[J].BMC Plant Biology,2006,6:27-32.
[17] 尹立荣,王军,宋润刚,等.山葡萄的花型遗传[J].园艺学报,1997,24(1):25-28.
[18] Antcliff A J. Inheritance of sex in Vitis[J].Ann Plant,1980,30:113-121.
[19] 王军,尹立荣,宋润刚,等.东北山葡萄花芽分化的研究[J].特产研究,1994,3:6-9.
[20] Elisabetta Caporali, Alberto Spada, Giovanna Marziani, et al. The arrest of development of abortive reproductive organs in the unisexual flower of Vitis vinifera ssp. Silvestris[J].Sex Plant Reprod,2003(15):291-300.

2个MADS-box 基因在山葡萄雌花和雄花中的表达分析

Two MADS-box Genes Expression Analysis in Male and Female Floral Development of Vitis amurensis Rupr.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	黄浩, 谢晋, 袁文彬, 王初亮, 陈坤华, 曾繁东, 梁增发, 苏诏, 王维. 不同有机物料对烤烟根系特征及氮磷钾积累量的影响[J]. 中国农学通报, 2022, 38(8): 51-57.
[2]	谷书杰, 钱禛锋, 娄永明, 沈庆庆, 普凤雅, 曾丹, 马豪, 何丽莲, 李富生. 接种内生菌对干旱胁迫下甘蔗的生理影响[J]. 中国农学通报, 2022, 38(6): 42-47.
[3]	韩敏, 杨鹏武, 何雨芩, 胡雪琼, 朱勇. 阴雨寡照灾害对云南烤烟的种植风险预测[J]. 中国农学通报, 2022, 38(4): 69-75.
[4]	关罗浩, 杨欣, 李超, 张艳玲, 张广富, 李永亮, 周汉平, 王广山, 钟帅, 戴华鑫. 留叶数对腾冲烟叶产质量的影响[J]. 中国农学通报, 2022, 38(4): 10-15.
[5]	黄磊, 李艺萌, 钟帅, 金保锋, 高晋军, 王晓园, 关罗浩, 王初亮, 黄妧婷, 李敏. 低氧贮存片烟解封后理化指标和质量变化研究[J]. 中国农学通报, 2022, 38(4): 107-112.
[6]	侯建林, 李思军, 何铭钰, 肖汉乾, 向铁军, 黄杰, 李武进, 肖艳松, 江智敏, 徐均华, 邓小华. 不同施肥模式对稻茬烤烟生长和干物质积累及产质量的影响[J]. 中国农学通报, 2022, 38(33): 39-43.
[7]	张志灵, 林海滨, 王新旺, 林隆, 陈星峰, 高伟民, 林祖斌, 陈庆文. 福建烤烟淀粉含量与外观质量和感官质量的关系[J]. 中国农学通报, 2022, 38(31): 136-141.
[8]	王月敏, 柯玉琴, 谢榕榕, 李春英, 李文卿. 喷施微肥对定位施肥烟株成熟期生理代谢的影响[J]. 中国农学通报, 2022, 38(31): 24-30.
[9]	樊仙, 全怡吉, 杨绍林, 李如丹, 邓军, 张跃彬. 甘蔗苗期抗旱性鉴定评价研究[J]. 中国农学通报, 2022, 38(3): 17-24.
[10]	陈检锋, 赵文军, 付利波, 尹梅, 王志远, 王伟, 王应学, 杨艳鲜, 陈华. 适产养分临界值模型对玉溪烤烟产质量的影响[J]. 中国农学通报, 2022, 38(28): 1-6.
[11]	董世良, 詹斯文, 何结望, 邱彤, 张雪琼, 刘峰峰. 基于HPLC指纹图谱技术的烤烟质量分类研究[J]. 中国农学通报, 2022, 38(25): 151-157.
[12]	王军, 田俊岭, 刘兰, 宗钊辉, 樊苗苗, 罗福命. 施肥水平对烤烟的干物质、养分积累及分配的影响[J]. 中国农学通报, 2022, 38(22): 8-14.
[13]	张艳艳, 代琛, 赵百英, 刘孟, 张本强, 武博, 高阳, 张强. 沂蒙丘陵生态区烤烟化学成分与感官质量的相关分析[J]. 中国农学通报, 2022, 38(22): 145-150.
[14]	刘静, 阳威, 成丹, 任永建, 王丽娟. 地表温度日较差对湖北烟区烤烟化学成分的影响[J]. 中国农学通报, 2022, 38(20): 97-104.
[15]	王晓春, 杨天辉, 王川, 杨炜迪, 高婷. 混合盐碱胁迫对紫花苜蓿的生长和生理指标的影响[J]. 中国农学通报, 2022, 38(19): 139-145.