欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 239-241.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

宁夏枣树品种品系的AFLP分析

魏天军1,李彦龙2,李百云1   

  • 收稿日期:2009-12-17 修回日期:2010-01-06 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:

    宁夏5183农业工程重大专项“枣树品种选育及无病毒苗木培育技术研究”中的部分内容。

AFLP analysis on jujube cultivars and strains in Ningxia Autonomous Region

Wei Tianjun1, Li Yanlong2, Li Baiyun1   

  • Received:2009-12-17 Revised:2010-01-06 Online:2010-05-20 Published:2010-05-20

PDF (PC)

868

摘要：

摘 要： 应用基因组DNA—AFLP分子标记技术，对原产宁夏回族自治区的8个枣树品种品系和原产甘肃靖远县的1个枣树品种的亲缘关系进行了研究。研究结果表明，5对引物（E1/M12、E1/M4、E4/M11、E2/M10和E5/M4）扩增出了466条带，其中多态性条带为130条，多态性比率达27.89%，表明遗传多样性不是很丰富。聚类结果证明灵武长枣2号与中卫大枣、同心圆枣聚为一类，彼此间近缘关系较近，而与灵武长枣品种的亲缘关系较远，是一个新种质；同心圆枣与中卫大枣的遗传距离仅为0.015，亲缘关系最近，是同物异名。

关键词: 胸腺素α1, 胸腺素α1, 大肠杆菌, 可溶性表达

Abstract:

Abstract：The genetic relationships among 9 jujube cultivars and strains were analyzed using the technique of genomic DNA-AFLP markers in Ningxia Hui People’s Autonomous Region. Five primer pairs (E1/M12、E1/M4、E4/M11、E2/M10和E5/M4) were used for the analysis. A total of 466 AFLP markers were identified, of which 130 were polymorphic, and polymorphism percentage was 27.89%. Using UPGMA(un-weighted pair group method arithmetic average) clustering method, 9 cultivars and strains were placed into 2 clusters. ‘Lingwuchangzao No.2’ selected from lingwuchangzao jujubes had farther genetic distance, that is a new germplasm of jujube. ‘Tongxinyuanzao’ jujube and ‘zhongweidazao’ jujube were very close genetically, being the same cultivar.