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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (2): 27-30.doi: 10.11924/j.issn.1000-6850.2014-0810

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

新疆伊犁某牛场不同生长期牛粪源大肠杆菌耐药性调查

郭庆勇1,南海辰1,夏利宁1,苏战强1,戴小华2   

  1. (1新疆农业大学动物医学学院,乌鲁木齐 830052；2新疆农业大学食品科学与药学学院,乌鲁木齐 830052）
  • 收稿日期:2014-03-24 修回日期:2014-12-14 接受日期:2014-05-15 出版日期:2015-03-19 发布日期:2015-03-19
  • 通讯作者: 夏利宁
  • 基金资助:
    国家自然科学基金项目“新疆猪源大肠杆菌耐药基因的流行性调查及其传播机制研究”(31260614)；新疆维吾尔自治区普通高校重点学科（基础兽医学）科研启动基金。

Survey of Resistance Escherichia coli Isolates to Antibiotics from Different Growth Stages Cattle in
A Cattle Farm in Ili, Xinjiang

Guo Qingyong1, Nan Haichen1, Xia Li’ning1, Su Zhanqiang1, Dai Xiaohua2   

  1. (1College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052;2College of Food Sciences and Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi 830052)
  • Received:2014-03-24 Revised:2014-12-14 Accepted:2014-05-15 Online:2015-03-19 Published:2015-03-19

摘要： 为了调查新疆伊犁某牛场不同生长期牛粪源大肠杆菌对临床常用抗菌药物的耐药情况。从该牛场分别采集育肥牛、繁育牛、加育牛和未繁育牛的粪样。采用微量肉汤稀释法，对分离出的大肠杆菌进行最小抑菌浓度测定。结果表明，育肥牛、繁育牛、加育牛和未繁育牛粪源大肠杆菌分离率均为100%，不同生长期的牛源大肠杆菌对氨苄西林均耐药，耐药率分别为4.0%、8.6%、6.7%、20.0%；对安普霉素都较为敏感。繁育牛和加育牛粪源大肠杆菌耐药种类较多，未繁育牛粪源大肠杆菌对比其他生长期牛粪源菌耐药较严重，不同生长期的牛粪源大肠杆菌多重耐药情况不太严重，主要为0耐、1耐；新疆伊犁该牛场对临床常用抗菌药物的耐药程度不同，耐药程度虽然不高，但在养殖生产中需注意合理用药。

关键词: 生理响应, 生理响应

Abstract: The study aims to investigate resistance of Escherichia coli isolates from cow dung at different cattle growth stages to commonly used antibiotics in a cattle farm in Ili, Xinjiang. Fecal samples were taken from fattening cattle, breeding cattle, strengthen fattening cattle and non-breeding cattle. Minimal inhibitory concentrations of the Escherichia coli isolates were determined by the broth micro-dilution method. Results showed that isolation rate of Escherichia coli from fattening cattle, breeding cattle, strengthen fattening cattle and non-breeding cattle were all 100%; Escherichia coli of different cattle growth stages showed resistance to ampicillin, resistance rates were 4.0%, 8.6%, 6.7% and 20.0%, respectively, and were more sensitive to apramycin. Resistant of Escherichia coli isolates of non-breeding cattle was more serious than that of the others. Multi-drug resistance of Escherichia coli isolates from cattle at different growth stages was less serious, and most of the isolates were resistant to zero and one antibiotic. While the level of drug resistance is not very high, rational drug application should be observed in production.