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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (14): 86-89.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

从甘蔗种茎茎汁中提取基因组DNA的方法

李建勇 蒙姣荣 陈保善   

  • 收稿日期:2010-03-12 修回日期:2010-04-16 出版日期:2010-07-20 发布日期:2010-07-20

Studies on the Methods of Extraction Genomic DNA from Sugarcane Seed-stem Juice

  • Received:2010-03-12 Revised:2010-04-16 Online:2010-07-20 Published:2010-07-20

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摘要： 摘 要： 为探索从甘蔗种茎茎汁中提取基因组DNA的有效方法，分别采用CTAB法、SDS-Ⅰ法和SDS-Ⅱ法进行提取，并以叶片为对照。对所提取的DNA质量进行了检测，结果表明：从茎汁中提取DNA时，SDS-Ⅰ法的产率最高。整体而言，种茎中提取到的DNA产率不如叶片高，但其浓度和纯度足以满足下游分子实验的要求，且其操作过程较叶片简便。

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Abstract: Abstract: Three different methods were used to extract genomic DNA from sugarcane seed-stem , and the methods were CTAB, SDS-Ⅰand SDS-Ⅱ. Leaves were taken as the control. The quality of the extracted DNA was detected. The results showed that the DNA yield of SDS-Ⅰwas the highest. Overall, although the yield of DNA from seed-stem was lower than leaves, but the concentration and purity of DNA from seed-stem were sufficient to meet the requirement of the downstream molecule experiments, and the operation was more convenient than taking leaves as the material.