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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (21): 278-281.

所属专题： 玉米

• 植物保护 农药 • 上一篇    下一篇

亚洲玉米螟优良球孢白僵菌菌株的筛选

徐艳聆 吕文彦 杜开书 王 蕊 张萌萌   

  • 收稿日期:2010-03-31 修回日期:2010-06-02 出版日期:2010-11-05 发布日期:2010-11-05
  • 基金资助:

    河南科技学院高学历人才科研基金

Screening of Superior Strain of Beauveria bassiana Parasitized on Ostrinia furnacalis

  • Received:2010-03-31 Revised:2010-06-02 Online:2010-11-05 Published:2010-11-05

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摘要：

本研究选择从亚洲玉米螟和桃蛀螟僵虫中分离纯化到的5个球孢白僵菌野生菌株，进行了毒力、菌株生长速率、产孢量和孢子萌发率的测定，筛选出了两株高毒力菌株B6和B17，在108孢子/mL浓度下对亚洲玉米螟的致死率分别为95.4％和90.8％，僵虫率分别为94.4％和87.8％，LT50分别为4.4d和4.7d；其LC50分别为1.3×106孢子/mL和2.8×106孢子/mL。而且这两株菌株显现出良好的生物学特性，具有较大生产潜力。

关键词: 酶抑制法, 酶抑制法, 假阳性, 辛辣蔬菜

Abstract:

Here five strains, isolated from the cadaver of corn borer and peach borer infected by Beauveria bassiana, were studied on virulence, growth, sporulation and germination. Two high virulent strains, B6 and B17, were obtained. The bioassay results showed that B6 and B17 had the high virulence on the corn borer with mortality of 95.4% and 90.8%, infected rate of 94.4% and 87.8％, median lethal concentration(LT50) of 4.4d and 4.7d at concentration of 108 spores/mL. The median lethal concentration(LC50)were 1.3×106 spores/mL and 2.8×106 spores/mL. The two strains had good biological characteristics too. Consequently, B6 and B17 strains have applied potentiality to control corn borer in future.