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中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 286-290.

• 植物保护 农药 • 上一篇    下一篇

漆树炭疽病病原鉴定及其生物学特性研究

江彤   

  • 收稿日期:2010-04-23 修回日期:2010-07-29 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    辅助离子注入选育高效生防菌对苹果采后炭疽病的控制效果与作用机理研究

Identification of Lacquer anthracnose pathogen and its biological characteristics research

  • Received:2010-04-23 Revised:2010-07-29 Online:2010-12-05 Published:2010-12-05

摘要:

采集感染炭疽病的漆树病叶,组织分离得病原菌纯培养,根据病原菌的形态、培养特征、致病性和寄主范围将其鉴定为胶孢炭疽Colletotrichum gloeosporioides (Penz.) Sacc.。进一步研究其生物学特性发现,漆树炭疽菌菌丝生长与时间呈直线相关,菌丝在10~35 ℃范围内均能生长,生长最适温度为25~28 ℃,分生孢子致死温度为48 ℃,分生孢子在相对湿度达到90 %时才能萌发,连续光照可以促进菌丝生长量和产孢量的增加,菌丝在pH 3~12 范围内均能生长,生长最适pH为6.0。漆树炭疽菌对多种碳、氮源都能利用,以葡萄糖、果糖为碳源以及以天门冬酰胺、丙氨酸为氮源时,其菌丝生长最佳。

关键词: 科学技术, 科学技术, 土地资源, 可持续发展

Abstract:

Lacquer leaves infected with anthracnose disease were collected, and pure culture of the pathogen was acquired by tissue isolation method. The pathogen is determined to be Colletotrichum gloeosporioides (Penz.) Sacc. by its morphologic characters, cultural characteristics, pathogenicity and host range. Further research work on its biological characteristics was conducted. It was found that mycelial growth of Lacquer anthracnose pathogen had a linear correlation with the cultural time. The temperature range of the mycelial growth is 10~35 ℃, and the optimum temperature is 25~28 ℃. The lethal temperature of conidia is 48℃. The conidia cannot germinate until relative humidity reaches 90%. Continuous light may promote the increasing of yield and sporulation of mycelia. The mycelia can grow at the range of pH 3~12, and the optimum growth pH is 6.0. The Lacquer anthracnose pathogen can utilize many kinds of carbon and nitrogen source. The mycelia will grow better if glucose, fruitose was used as the carbon sources and asparamide, alanine was used as the nitrogen sources.