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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (1): 342-345.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

贵州省2个猪种APOA5基因比较分析

杨远青 李敬瑞 惠嫣婷 刘若余   

  • 收稿日期:2010-05-21 修回日期:2010-06-23 出版日期:2011-01-07 发布日期:2011-01-07
  • 基金资助:

    贵州省农业科技攻关项目.

Comparing Analysis of APOA5 Gene of Two Pigs Species in Guizhou Province

  • Received:2010-05-21 Revised:2010-06-23 Online:2011-01-07 Published:2011-01-07

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摘要：

摘 要：根据GenBank中报道的猪载脂蛋白A5 (apoA5)基因序列设计3对引物，通过PCR方法分别对可乐猪和贵州白香猪的apoA5基因进行扩增、测序，将两个猪种apoA5基因序列进行比对，并找出基因变异位点。结果表明：可乐猪apoA5基因DNA长为2474bp，白香猪apoA5基因DNA长为2473bp，分别包含三个外显子，两个内含子。两猪种apoA5基因编码区长皆为1092bp，编码363个氨基酸。白香猪apoA5基因序列与可乐猪相比，存在12个突变位点，其中6个位点为颠换，5个为转换，1个为缺失，两者核苷酸同源性为99.6%。这为进一步研究猪apoA5基因的多态性、生物学活性以及与猪生产性状相关性研究奠定了一定基础

关键词: 低温胁迫, 低温胁迫, 玉米, 根系

Abstract:

Abstract:According to pig apolipoprotein A5 (apoA5) gene sequence in GenBank，we design 3 pairs of primers for amplification of Kele pig and Guizhou White Xiang pig′s apoA5 gene respectively by PCR and sequencing, and compare the two pigs species apoA5 gene sequences to identify the genetic variation. The results showed: Kele pig apoA5 gene DNA length is 2474bp.White Xiang pig gene apoA5 gene DNA length is 2473bp,which contains three exons and two introns respectively. ApoA5 gene coding region in both of the two pig breeds are 1092bp encoding 363 amino acids. Compared the White Xiang pig apoA5 sequence with the Kele′s, there are 12 mutations, including six transversion,five transition,one deletion mutation, the two pig′s nucleotide homology was 99.6%. It provids a certain foundation for the further study of pig apoA5 gene polymorphism, biological activity and the pig′s production traits。