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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (3): 337-341.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

2009年山东H9N2亚型禽流感M基因的分子进化分析及对哺乳动物致病性的研究

朱雯迎 许传田 崔言顺 鲁梅 颜世敢 胡北侠 杨少华 李建亮 任海松 张秀美   

  • 收稿日期:2010-08-09 修回日期:2010-11-05 出版日期:2011-02-05 发布日期:2011-02-05
  • 基金资助:

    山东省博士基金 现代农业产业技术体系建设专项资金资助

Evolution Analysis of the Matrix (M) Protein Genes of H9N2 Chicken Influenza Viruses Isolated in Shandong in 2009 and the Pathgenicity to Mammal

  • Received:2010-08-09 Revised:2010-11-05 Online:2011-02-05 Published:2011-02-05

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摘要：

根据GenBank公布的H9N2亚型禽流感病毒(AIV)M基因序列设计引物,通过反转录-聚合酶链式反应(RT-PCR)方法扩增H9N2亚型禽流感病毒M基因，并与GenBank中AIV M基因序列进行了同源性比较和氨基酸编码分析,绘制了M基因的系统发育进化树。结果表明：这6株病毒的M基因的核苷酸同源性在95.9-99.4%。M1蛋白的同源性在97.6-99.6%。M2蛋白的同源性在95.1-100%。进化分析这6株病毒都属于A/Quail/Hong- Kong/G1/97 (G1)-like 分支。这6株病毒的M2蛋白的第31位氨基酸存在S→N（AGT→AAT）的变异，都具有金刚烷胺的抗性。以106 EID50 的剂量，将H9N2 病毒鼻腔感染BALB/c 小鼠，结果表明H9N2 亚型流感病毒可以对哺乳动物小鼠可使得体重下降，也可致死。.本研究结果对于揭示H9N2 亚型禽流感流行规律和病毒的致病机理具有一定的意义。

关键词: 毛白杨, 毛白杨, 造林地, 速效磷, 速效钾, 空间变异

Abstract:

The Matrix (M) genes of 6 H9N2 subtype avian Influenza Viruses (AIVs)which isolated from chickens in Shandong in 2009 , were sequenced and molecular evolutionarily analyzed in this study.Homology of nucleotide sequences in the M gene of 6 isolates was 95.1–99.4%. The identity among the M1 protein was 97.6–99.6% while the M2 protein was 95.1–100%. Phylogenetic analysis showed that all of the tested M genes belong to the A/Quail/Hong- Kong/G1/97 (G1)-like lineage. An analysis of the viral amino acid sequence of M2 protein has revealed substitution of S31N in two isolates, which is the molecular characterization of amantadine resistance in AIVs. The BALB/c mice aged 6 weeks with 106 EID50 H9N2, lost weight progressively, and some died within 7 days of infection. Results of this study suggest that long-term monitoring should be continued to track the transmission and evolution of H9N2 AIVs in chickens in China.

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