欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (5): 312-316.

所属专题： 生物技术； 烟草种植与生产

• 生物技术科学 • 上一篇    下一篇

烟草CMS相关基因orf25的生物信息学分析

周 玮 黄 河 周伯楠 刘齐元   

  • 收稿日期:2010-09-15 修回日期:2010-10-26 出版日期:2011-03-05 发布日期:2011-03-05
  • 基金资助:

    烟草线粒体ORF25与CMS的关系;新质源烟草胞质雄性不育相关基因的克隆

The Bioinformatics Analysis of orf25 Gene Related to Tobacco Cytoplasmic Male Sterility

  • Received:2010-09-15 Revised:2010-10-26 Online:2011-03-05 Published:2011-03-05

PDF (PC)

1079

摘要：

线粒体基因orf25异常可能导致烟草细胞质雄性不育（CMS）。为探讨orf25基因导致CMS的可能机理，本文采用生物信息学方法，分析烟草雄性不育系‘MS革新3号’及其保持系中的orf25基因在其编码蛋白各结构层次上存在的差异。结果显示，不育系中orf25基因因碱基改变提前出现终止子从而导致其编码蛋白缺失结构域，这极可能成为干扰线粒体F(0)F(1)-ATP合成酶功能的关键因素，从而成为导致烟草‘革新3号’CMS的根本原因之一。

关键词: 干物质积累, 干物质积累

Abstract:

It was reported that aberrant mitochondrial gene orf25 might cause tobacco cytoplasmic male sterility (CMS). To understand the mechanism of CMS, the differences of orf25 gene between CMS line ‘Gexin 3’ and its maintainer line were analyzed by bioinformatics at different structure levels of coding protein. The results showed that the terminal codon appeared early because of the base change in the orf25 gene, which led to the lack of the structural domain in coding protein. It might be the key factor that disturbed the function of F(0)F(1)-ATP synthetase in mitochondrion, and become one of the fundamental causes which led to CMS of tobacco.