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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (4): 321-325.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

一株产胶原酶海洋微生物发酵条件的研究

白杨 钱斯日古楞 王红英   

  • 收稿日期:2010-09-15 修回日期:2010-11-04 出版日期:2011-02-10 发布日期:2011-02-10
  • 基金资助:

    辽宁省教育厅A类项目

Fermentation Conditions of a Collagenase-Producing Marine Microorganism

  • Received:2010-09-15 Revised:2010-11-04 Online:2011-02-10 Published:2011-02-10

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摘要：

于海洋污泥中筛选得到的Aeromonas sp.F3所产的胞外酶对胶原蛋白有水解作用，采用单因素试验法对Aeromonas sp.F3的发酵条件及培养基组分进行全面优化，提高该胶原酶生产菌的产酶效率。经优化试验结果表明，该菌株最佳发酵条件为：培养时间24 h，温度40℃，初始pH为8，装瓶量80~100 mL/250 mL；培养基组分为：葡萄糖3 g/L，酵母浸粉9 g/L，NaCl 5 g/L，MnSO4 10 mg/L。经优化试验明显提升了该菌株的产酶能力。

关键词: 乡村旅游, 乡村旅游, 游客旅游行为特征, 发展建议, 双芹村, 福建沿海地区

Abstract:

Aeromonas sp.F3 screened from marine mud should secrete a protease to hydrolyze collagen. The fermentation of Aeromonas sp.F3 was investigated through single-factor test. The main factors included fermentation conditions and the medium. With single factor trial, the optimal culture condition was determined: 40℃ of fermentation temperature, pH8 and volume 80~100 mL/250 mL for 24 h. The optimal liquid medium was: 3 g/L glucose as carbon source, 9 g/L yeast extract powder as nitrogen source, 5 g/L NaCl as inorganic salt and 10 mg/L MnSO4 as metal ion. Under the optimum condition, the enzyme activity increased very observably.