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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (5): 338-345.

• 植物保护 农药 • 上一篇    下一篇

长枝木霉TlCC鉴定及其生物学特性研究

王斌 Ali Khatib Bakar 刘金亮 张世宏 潘洪玉   

  • 收稿日期:2010-10-19 修回日期:2010-11-21 出版日期:2011-03-05 发布日期:2011-03-05
  • 基金资助:

    国家科技支撑计划项目;吉林省科技发展计划项目;吉林大学“211工程”建设项目

The Study on Identification and Biological Characteristics of Trichoderma longibrachiatum TlCC

  • Received:2010-10-19 Revised:2010-11-21 Online:2011-03-05 Published:2011-03-05

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摘要：

从土壤中分离得到1株木霉TlCC，为明确其分类地位及其生物学特性，对该菌进行形态学鉴定和分子鉴定，并对其生长培养基、光照、温度、碳源、氮源、微量元素和pH等生物学特性进行研究。鉴定结果显示，该菌株为长枝木霉（Trichoderm longibrachiatum）。生物学特性结果表明：菌株TlCC在PDA上生长量最大，查氏培养基和基本培养基上生长量最小；不同光照条件下，菌丝生长无明显差异，但在连续光照条件下可明显促进孢子产生；在15~40℃范围能够生长产孢，在35℃时菌丝生长最快，但产孢量在25~35℃最大；果糖最能促进菌丝生长，果糖、可溶性淀粉最有利于产孢；谷氨酸对菌丝生长和孢子产生都有促进作用；Cu最能促进菌丝生长，而Zn、K和Mg则对菌丝生长有一定抑制作用，Cu最有利于产孢，Zn对产孢明显抑制；在pH值在2~10范围内均可生长，在pH为5时为生长最佳，在pH值3~7范围内最有利于产孢。

关键词: 蛋壳颜色, 蛋壳颜色

Abstract:

A Trichoderma isolated from soil (TlCC) was identified as Trichoderma longibrachiatum on the basis of their morphology and DNA sequence analysis of ITS rDNA. The biological characteristics of TlCC were screened and investigated. Though it could grow on PSA, PMA, NA, Czapek and basic cultrure medium, PDA was the most favorable medium for the hypha growth. It could grow and produce spore from 15℃ to 40℃, but the optimal temperature for the growth was 35℃, and for the sporulation was from 25℃ to 35℃. For the basic culture medium, fructose was optimal carbon source for hypha growth, fructose and tragantine were propitious to spore production. The best nitrogen source for hypha growth and spore production was glutamate. The pH for hyphae growth and spore production ranged between 2 and 10, the optimal pH for hyphae growth was 5, from 3 to 7 were propitious to spore production.

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