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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 269-272.

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

3种方法检测黄瓜绿斑驳花叶病毒（CGMMV）的灵敏度对比分析

李海明 沈建国 吴祖建 陈启建   

  • 收稿日期:2010-03-22 修回日期:2010-04-29 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    鸦胆子素D抗烟草花叶病毒机制研究;外来有害生物预警与监测技术体系研究;福建出入境检验检疫局科技项目

Analysis on comparison of sensitivities of three detecting techniques of Cucumber Green Mottle Mosaic Virus

  • Received:2010-03-22 Revised:2010-04-29 Online:2010-09-05 Published:2010-09-05

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摘要：

本研究以黄瓜绿斑驳花叶病毒侵染的黄瓜叶片为材料，应用双抗体夹心酶联免疫吸附测定法（DAS-ELISA）、反转录-聚合酶链反应（RT-PCR）、核酸斑点杂交（NASH）技术，进行了检测灵敏度比较研究。三种检测方法灵敏度实验结果表明：RT-PCR检测灵敏度高于地高辛标记的核酸斑点杂交技术，以血清学为基础的DAS-ELISA检测灵敏度最低。通过灵敏度对比分析得出，核酸斑点杂交技术可以替代RT-PCR应用于基层的检疫检测。

关键词: 控释BB肥, 控释BB肥, 脲酶, 中性磷酸酶, 蔗糖酶

Abstract:

Cucumber green mottle mosaic virus in virus-infected cucumber leaves was detected by DAS-ELISA、RT-PCR and NASH techniques and the sensitivities of detection were compared after the total RNA extracted from 1g virus-infected leaves were diluted gradient 。The results showed that the detection of RT-PCR was the most sensitive; NASH was more sensitive than DAS-ELISA. NASH technique can be replaced of RT-PCR used in metrical quarantine departments by comparison of sensitivities of three detect techniques