基于Gateway技术的拟南芥雄性不育GHF基因表达载体的构建

摘要/Abstract

摘要： 随着模式植物拟南芥全基因组测序工作的完成更多雄性不育相关基因功能需要阐明。利用拟南芥突变体库，从反向遗传学角度进行突变基因的功能研究需要构建相应的载体进行互补实验。Gateway技术是一种高效，快速的克隆系统。笔者利用Gateway克隆技术成功构建拟南芥GHF基因表达载体，并遗传转化拟南芥，为进一步阐明该基因功能奠定了基础。

关键词: 肌醇磷脂, 肌醇磷脂, 渗透胁迫, 磷酸肌醇激酶, ABA

Abstract: With the completion of the whole genome sequence of mode plant Arabidopsis, more functions of male sterile gene need to be expounded. Using Arabidopsis mutant bank to identify functions of mutant gene and corresponding vector need to be constructed .In order to carry out the complementary experiment. Gateway cloning technology is a fast cloning system. In this article, using Gateway technology GHF gene expression vector was constructed and introduced into Arabidopsis. It was useful for further gene functional research.

中图分类号:

胡金艳,,张书祥,王晓武,武剑. 基于Gateway技术的拟南芥雄性不育GHF基因表达载体的构建[J]. 中国农学通报, 2007, 23(12): 45-45.

Hu jinyan,, Zhang shuxiang, Wang xiaowu, Wu jian. Construction of expression vector of Arabidopsis Male sterile GHF gene using Gateway Cloning Technology[J]. Chinese Agricultural Science Bulletin, 2007, 23(12): 45-45.

参考文献

[1] Bushman W,Thompson J F,Vargas L et al. Control of directionality in lambda site specific recombination. Science,1985,230(4278):906-911.
[2] Landy A. Dynamic,structural,and regulatory aspects of lambda site-specific recombination. Annu Rev Biochem,1989,58:913-949.
[3] Hartley J L,Temple G F,Brasch M A. DNA cloning using in vitro site-specific recombination. Genome Research,2000,10(11):1788-1795.
[4] Meyerowitz EM. Arabidopsis thaliana. Annu Rev Genetics,1987,21:93-111.
[5] Koncz C, Chua N-H, Schell J. Methods in Arabidopsis Research. World Scientific Press,1992.
[6] Kaul S, Koo H L, Fraser C M et al. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature,2000,408:796-815.
[7] Sanders PM, Bui AQ, Weterings K et al. Anther developmental defects in Arabidopsis thaliana male-sterile mutants. Sex Plant Reprod,1999,11:297-322.
[8] Wilson ZA, Morroll SM, Dawson J et al. The Arabidopsis male sterility 1 (MS1) gene is a transcriptional regulator of male gametogenesis, with homology to the PHD-finger family of transcription factors．Plant J,2001,28:27-39.
[9] Azpiroz-Leehan R, Feldmann K. T-DNA insertion mutagenesis in Arabidopsis:Going back and forth. Trends Genet,1997,13:152-156.
[10] Krysan P J, Young J C, Sussman M R. T-DNA as an insertional mutagen in Arabidopsis. Plant Cell,1999,11:2283-2290.
[11] Patrick J Krysan, Jeffery C Young, Michael R. T-DNA as an Insertional Mutagen in Arabidopsis. Plant Cell,1999,11:2283-2290.
[12] Clough S J,Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J ,1998,16:735-743.
[13] Den Dulk-Ras A, Hooykaas P J J. Electroporation of Agrobacterium tumefaciens. Methods Mol. Biol,1995,55:63-72.
[14] Walhout A J, Sordella R, Lu X et al. Protein interaction mapping in C.elegans using proteins involved in vulval development. Science,2000,287(5450):116-122.
[15] Rudolph C, Schreier P H, Uhrig J F. Peptide-mediated broad-spectrum plant resistance to tospoviruses. Proc Natl Acad Sci,2003,100(8):4429-4434.
[16] Grand-Perret T, Bouillot A, Perrot A et al. SCAP ligands are potent new lipid-lowering drugs. Nat Med,2001,7(12):1332-1338.
[17] 陈其军,安瑞,周海梦,等.使用与Gateway技术兼容的T载体获得入门克隆.生物化学与生物物理进展,2004,31(10):951-954.
[18] 梅文倩,宋文强,朱玉贤,等.利用Gateway克隆技术大规模克隆拟南芥转录因子.分子植物育种,2004,2(3):358-364.
[19] Bernard P, Couturier M. Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase Ⅱ complexes. Mol Bio,1992,226(3):735-745.