孕穗期低温条件下ABA对水稻结实率及叶片生理特性的影响

doi:10.11924/j.issn.1000-6850.casb16070120

中国农学通报 ›› 2016, Vol. 32 ›› Issue (36): 16-23.doi: 10.11924/j.issn.1000-6850.casb16070120

所属专题：生物技术；水稻

孕穗期低温条件下ABA对水稻结实率及叶片生理特性的影响

项洪涛^1,2,王彤彤²,郑殿峰³,王立志²,洛育²,李琬^1,2

(¹黑龙江省农业科学院博士后工作站,哈尔滨 150086；²黑龙江省农业科学院耕作栽培研究所,哈尔滨 150086；³黑龙江八一农垦大学,黑龙江大庆 163319）

收稿日期:2016-07-24 修回日期:2016-09-19 接受日期:2016-09-21 出版日期:2016-12-26 发布日期:2016-12-26
通讯作者: 项洪涛
基金资助:
中国博士后基金面上项目“开花期低温导致寒地粳稻颖花败育的生理学机理研究”(2016M591563)；黑龙江省政府博士后资助基金项目“花期低温导致水稻颖花败育的机理研究”(LBH-Z15197)；黑龙江省科学基金面上项目“黑龙江省水稻抗瘟模块的挖掘及近等基因系构建”(C2015028)；黑龙江省农业科技创新工程青年基金项目“ABA对水稻障碍型冷害防控效果研究”(2013QN005)；黑龙江省农业科学院引进博士人员科研启动金项目“孕穗期低温导致水稻颖花败育的机理研究”(201507-07)。

Effect of ABA on Seed-setting Rate and Physiological Characteristics of Rice Leaves Under Low Temperature Stress at Booting Stage

Xiang Hongtao^1,2, Wang Tongtong², Zheng Dianfeng³, Wang Lizhi², Luo Yu², Li Wan^1,2

(¹Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086; ²Crop Tillage and Cultivation Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086; ³Collage of Agronomy, Heilongjiang Bayi Agricultural University, Daqing Heilongjiang 163319)

Received:2016-07-24 Revised:2016-09-19 Accepted:2016-09-21 Online:2016-12-26 Published:2016-12-26

摘要/Abstract

摘要： 为了明确ABA对孕穗期低温下水稻结实率及叶片生理特性的调控效应，本试验在盆栽条件下，以2个水稻(Oryza sativa L.)品种‘龙粳11’（冷敏型）和‘龙稻5’（耐冷型）为材料，于孕穗期在人工气候室进行低温（15℃，持续1，2，3，4，5天）处理，并喷施不同浓度的脱落酸(ABA)，研究低温条件下ABA对不同水稻结实率的调控效应，同时探讨了ABA和低温对水稻叶片膜透性、抗氧化酶等生理特性的影响。结果表明：低温条件下，‘龙稻5’的结实率变化不大，‘龙粳11’的结实率显著降低，20 mg/L的ABA能够有效缓解低温伤害，相对提高低温胁迫下水稻的结实率。叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性表现出先升高后下降的变化趋势，‘龙稻5’的抗氧化酶活性、可溶性糖和可溶性蛋白含量高于‘龙粳11’，但丙二醛(MDA)含量和相对电导率低于‘龙粳11’。ABA可提高冷敏品种的抗氧化酶活性、可溶性糖和可溶性蛋白的含量，同时可降低MDA含量和相对电导率。这说明ABA可以通过调控水稻抗氧化酶、可溶性糖等生理指标的活性及含量，进而提高冷敏水稻品种的耐冷性。

关键词: 糖, 糖, 根系, 生长发育, 调控机理

Abstract: The aim is to clarify the regulating effects of ABA under low temperature stress on seed-setting rate and physiological characteristics of rice leaves at booting stage. This study was conducted with two rice cultivars ‘Longjing11’ (cold sensitive) and ‘Longdao5’ (cold tolerant) which were planted in pots. At booting stage, the low temperature treatments (15℃, lasting 1, 2, 3, 4, 5 d) were carried out in climatic laboratory. The different concentrations of abscisic acid (ABA) were sprayed. The regulating effects of ABA on the seed-setting rate of different rice cultivars were studied. The effects of ABA and low temperature on the membrane permeability of leaf and antioxidant enzyme were also discussed. The results showed that the seed-setting rate of ‘Longdao5’ had little change in low temperature condition, and the seed-setting rate of ‘Longjing11’ decreased significantly. Spraying of 20 mg/L ABA could effectively alleviate the cold damage, and the seed-setting rate was improved relatively under low temperature. The activities of SOD, POD and CAT in leaves first showed an increasing trend then a decreasing trend. The activities of antioxidase, contents of soluble sugar and soluble protein of ‘Longdao5’ were higher than those of ‘Longjing11’, but the content of malondialdehyde (MDA) and relative conductivity were lower than that of ‘Longjing11’. It indicated that ABA could increase the cold tolerance of cold sensitive rice cultivar through regulating some physiological characteristics such as the activities of antioxidant enzyme, the content of soluble sugar in leaf.

项洪涛,王彤彤,郑殿峰,王立志,洛育,李琬. 孕穗期低温条件下ABA对水稻结实率及叶片生理特性的影响[J]. 中国农学通报, 2016, 32(36): 16-23.

Xiang Hongtao,Wang Tongtong,Zheng Dianfeng,Wang Lizhi,Luo Yu and Li Wan. Effect of ABA on Seed-setting Rate and Physiological Characteristics of Rice Leaves Under Low Temperature Stress at Booting Stage[J]. Chinese Agricultural Science Bulletin, 2016, 32(36): 16-23.

参考文献

[1] Mamun E A, Cantrill L C, Overall R L, et al. Mechanism of low-temperature-induced pollen failure in rice[J]. Cell Biology International, 2010,34(5):469-476.
[2] De Datta S K. Principles and Practices of Rice Production[M]. New York: Robert E. Krieger publishing company, 1981.
[3] Renata Pereira da Cruz, Raul Antonio Sperotto, Denise Cargnelutti, et al. Avoiding damage and achieving cold tolerance in rice plants[J]. Food and Energy Security, 2013,2(2):96-119.
[4] Cheng C, Yun K Y, Ressom H W, et al. An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice[J]. BMC Genomics, 2007, 8:175.
[5] Oliver S N, Dennis E S, Dolferus R. ABA Regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice[J]. Plant Cell Physiol, 2007,48:1319-1330.
[6] Je′ro?me Verdier, Richard D. Thompson transcriptional regulation of storage protein synthesis during dicotyledon seed filling[J]. Plant Cell Physiol, 2008, 49(9):1263-1271.
[7] Kende H, Zeevaart J A D. The five “classical” plant hormones[J]. Plant Cell, 1997,9:1197-1210.
[8] Sansberro P, Mroginski L, Bottini R. Abscisic acid promotes growth of Ilex paraguariensis plants by alleviating diurnal water stress[J]. Plant Growth Regulation, 2004,42:105-111.
[9] Peng Y B, Zou C, Wang D H, et al. Preferential localization of abscisic acid in primordial and nursing cells of reproductive organs of Arabidopsis and Cucumber[J]. New Phytologist, 2006,170(3):459-466.
[10] Travaglia C, Reinoso H, Bottini R. Application of abscisic acid promotes yield in field-cultured soybean by enhancing production of carbohydrates and their allocation in seed[J]. Crop & Pasture Science, 2009, 60:1131-1136.
[11] 席吉龙,张建诚,席凯鹏,等.外源ABA对小麦抗旱性和产量性状的影响[J].作物杂志,2014,3:105-108.
[12] 王远敏,王光明.ABA浸种对水稻生长发育及产量的效应研究[J].西南师范大学学报:自然科学版,2007,32(1):91-96.
[13] 曾卓华,易泽林,王光明,等.ABA浸种对水稻幼苗生理及产量性状的影响[J].西南大学学报:自然科学版,2009,31(10):52-56.
[14] 邵玺文,孙长占,阮长春,等.ABA浸种对水稻生长及产量的影响[J].吉林农业大学学报,2003,25(3):243-245.
[15] 黄宇,苏以荣,谢小立,等.ABA对双季早稻产量的影响[J].湖南农业科学,2001,(1):23-24.
[16] 李晶,张丽芳,焦健,等.低温胁迫下外源ABA对玉米幼苗生长的影响[J].东北农业大学学报,2015,46(11):1-7.
[17] 方彦,武军艳,孙万仓,等.外源ABA浸种对冬油菜种子萌发及幼苗抗寒性的诱导效应[J].干旱地区农业研究,2004,32(6):70-74.
[18] 莫小锋,贲柳珍,邱莉维,等.外源ABA处理提高结缕草抗寒性试验[J].南方园艺,2014,25(3):6-10.
[19] 王军虹,徐琛,苍晶,等.外源ABA对低温胁迫下冬小麦细胞膜脂组分及膜透性的影响[J].东北农业大学学报,2014,45(10):21-28.
[20] 李宁,王萍,李烨,等.外源化学物质对低温胁迫下茄子细胞膜系统的影响[J].长江蔬菜,2012(6):20-22.
[21] 周碧燕,郭振飞.ABA及其合成抑制剂对柱花草抗冷性及抗氧化酶活性的影响[J].草业学报,2005,14(6):94-99.
[22] 金喜军,宋柏全,杨君凯,等.GA和ABA对甜菜幼苗保护酶活性的影响[J].中国糖料,2015,37(4):20-23.
[23] 杨东清,王振林,尹燕枰,等.外源ABA和6-BA对不同持绿型小麦旗叶衰老的影响及其生理机制[J].作物学报,2013,39(6):1096-1104.
[24] Zhang Y, Jiang W J, Yu H J, et al. Exogenous abscisic acid alleviates low temperature-induced oxidative damage in seedlings of Cucumis sativus.L[J]. Transactions of the Chinese Society of Agricultural Engineering, 2012,28(Supp.2): 221-228.
[25] 郭贵华,刘海艳,李刚华,等.ABA缓解水稻孕穗期干旱胁迫生理特性的分析[J].中国农业科学,2014,47(22):4380-4391.
[26] 黄凤莲,戴良英,罗宽.药剂诱导水稻幼苗抗寒机制研究[J].作物学报,2000,26(1):92-97.
[27] 李合生,孙群,赵世杰,等.植物生理生化试验原理和技术[M].北京:高等教育出版社,2000:167-169;184-185.
[28] 张宪政.作物生理研究法[M].北京:农业出版社,1992:201-202.
[29] 夏楠,赵宏伟,吕艳超,等.灌浆结实期冷水胁迫对寒地粳稻籽粒淀粉积累及相关酶活性的影响[J].中国水稻科学,2016,30(1):62-74.
[30] Howell K A, Narsai R, Carroll A, et al. Mapping metabolic and transcript temporal switches during germination in rice highlights specific transcription factors and the role of RNA instability in the germination process[J]. Plant physiology, 2009, 149:961-980.
[31] Morsy M R, Jouve L, Hausman J F, et al. Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance[J]. Journal of plant physiology, 2007,164:157-167.
[32] Jacobs B C, Pearson C J. Growth, development and yield of rice in response to cold temperature[J]. J. Agro. Crop Sci.,1999,182:79-88.
[33] Oda S, Kaneko F, Yano K, et al. Morphological and gene expression analysis under cool temperature conditions in rice anther development[J]. Genes and genetic systems, 2010, 85:107-120.
[34] 王连敏.寒地水稻耐冷基础的研究(Ⅱ):小孢子阶段低温对水稻结实的影响[J].中国农业气象, 1997,18(4):10-21.
[35] Anderson M D, Prasad T K, Martin B A, et al. Differential gene expression in chilling-acclimated maize seedlings and evidence for the involvement of ABA in chilling tolerance[J]. Plant Physiol., 1994,105:331-339.
[36] 王立志,项洪涛,王连敏,等.不同水稻品种对孕穗期低温的敏感性分析[J].黑龙江农业科学,2014(9):14-17.
[37] 朱海霞,王秋京,闫平,等.孕穗抽穗期低温处理对黑龙江省主栽水稻品种结实率的影响[J].中国农业气象,2012,33(2):304-309.
[38] 曾宪国,项洪涛,王立志,等.孕穗期不同低温对水稻空壳率的影响[J].黑龙江农业科学,2014(6):19-21.
[39] Gowing D J, Davies W J, Johes H G. Apositiveroot-sourced signal as an“indicator” of soil drying in apple[j]. J ExpBot,1990,41(233):1535-1540.
[40] 王玉霞.脱落酸（ABA）对亚种间杂交稻籽粒发育及蔗糖合酶活性的调节机理研究[D].扬州:扬州大学,2007
[41] 杨建昌,彭少兵,顾世梁,等.水稻灌浆期籽粒中3个与淀粉合成有关的酶活性变化[J].作物学报,2001,27(2):157-164.
[42] Reiko Shinkawa, Aiko Morishita, Kumiko Amikura, et al. Abscisic acid induced freezing tolerance in chilling-sensitive suspension cultures and seedlings of rice[J]. BMC Research Notes, 2013, 6:351-364.
[43] Mantyla E, Lang V, Palva E T. Role of abscisic acid in drought-induced freezing tolerance, cold acclimation and accumulation of LTI78 and RAB18 proteins in Arabidopsis thaliana[J]. Plant Physiol, 1995,107:141-148.
[44] 姜卫东,高光林,俞开锦,等.水分胁迫对果树光合作用及同化代谢的影响研究进展[J].果树学报,2002,19(6):416-420.
[45] 王英哲,任伟,徐安凯,等.低温胁迫下紫花苜蓿对外源SA和ABA的生理响应[J].华北农学报,2012,27(5):144-149.
[46] 苗永美,王万洋,杨海林,等.外源Ca²⁺、SA和ABA缓解甜瓜低温胁迫伤害的生理作用[J].南京农业大学学报,2013,36(4):25-29.
[47] 张桂莲,张顺堂,肖浪涛,等.抽穗开花期高温胁迫对水稻花药、花粉粒及柱头生理特性的影响[J].中国水稻科学,2014,28(2):155-166.
[48] 刘祖琪,张石城.植物抗性生理学[M].北京:中国农业出版社,1994:43-44.
[49] 简令成.植物冻抗寒机理研究的新进展[J].植物学通报,1992,9(3):17-22.
[50] 黄杏,陈明辉,杨丽涛,等.低温胁迫下外源ABA对甘蔗幼苗抗寒性及内源激素的影响[J].华中农业大学学报,2013,32(4):6-11.
[51] 陈善娜,郭浙红,沈云光,等.在低温胁迫下外源ABA对高原水稻自由基清除系统的影响[J].云南大学学报:自然科学版,1996,18(2):167-172.

孕穗期低温条件下ABA对水稻结实率及叶片生理特性的影响

Effect of ABA on Seed-setting Rate and Physiological Characteristics of Rice Leaves Under Low Temperature Stress at Booting Stage

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	高文瑞, 孙艳军, 韩冰, 费聪, 王显生, 徐刚. 弱光对西瓜果实品质及蔗糖代谢的影响[J]. 中国农学通报, 2023, 39(1): 56-61.
[2]	武迪, 张锋, 隋春莹, 师君慧, 万雪洁, 刘义国, 韩伟, 师长海. 外源活性物质对小麦苗期抗逆性的影响[J]. 中国农学通报, 2022, 38(9): 14-19.
[3]	陈英花, 白如霄, 王娟, 张新疆, 刘玲慧, 刘小龙, 冯国瑞, 危常州. 叶面喷施烯效唑和硼对塔额盆地甜菜产量和含糖率的影响[J]. 中国农学通报, 2022, 38(9): 41-48.
[4]	孙歌, 接伟光, 胡崴, 张颖智, 乔巍, 魏丽娜, 姜怡彤, 白莉. 菌根真菌及菌根辅助细菌对农作物发育的影响研究进展[J]. 中国农学通报, 2022, 38(9): 88-92.
[5]	黄浩, 谢晋, 袁文彬, 王初亮, 陈坤华, 曾繁东, 梁增发, 苏诏, 王维. 不同有机物料对烤烟根系特征及氮磷钾积累量的影响[J]. 中国农学通报, 2022, 38(8): 51-57.
[6]	邓裕帅, 王宇光, 於丽华, 耿贵. 水涝胁迫对不同土壤盐碱度下甜菜幼苗生长及光合特性的影响[J]. 中国农学通报, 2022, 38(7): 18-23.
[7]	李乐, 张晓虎, 杨澜. 黄芩茎叶没食子酸-KGM复合保鲜膜在鸡蛋保鲜中的应用研究[J]. 中国农学通报, 2022, 38(35): 125-133.
[8]	徐翎清, 李佳佳, 常晓, 张云龙, 刘大丽. 土壤氮矿化相关机理的研究进展[J]. 中国农学通报, 2022, 38(34): 97-101.
[9]	于亮亮, 孔德胤, 高培德, 包佳婧, 孔鸣川. 华莱士瓜含糖率农业气象指标与气候品质评价[J]. 中国农学通报, 2022, 38(34): 102-106.
[10]	查倩, 奚晓军, 殷向静, 蒋爱丽. 转色期补光处理对鲜食葡萄‘巨峰’和‘巨玫瑰’果实品质的影响[J]. 中国农学通报, 2022, 38(31): 55-59.
[11]	刘永惠, 沈一, 沈悦, 梁满, 陈志德. 花生籽仁发育过程中糖分积累特征及蔗糖代谢酶活性分析[J]. 中国农学通报, 2022, 38(30): 29-34.
[12]	董寅壮, 王堽, 於丽华, 耿贵. 亚铁胁迫对甜菜幼苗矿质元素积累的影响[J]. 中国农学通报, 2022, 38(3): 11-16.
[13]	隋振全, 范金石, 尹崇山, 毛金超. 壳聚糖对植物病原体的作用机制及其影响因素[J]. 中国农学通报, 2022, 38(3): 121-126.
[14]	吴文. 切根对乌桕容器苗质量的影响[J]. 中国农学通报, 2022, 38(3): 25-29.
[15]	王超, 杨长耀, 李菲菲, 马小川, 陈岳文, 肖志伟, 尹韬, 叶丽, 李云松, 卢晓鹏. 柑橘CsaccA基因功能鉴定[J]. 中国农学通报, 2022, 38(28): 28-35.