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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (15): 102-106.doi: 10.11924/j.issn.1000-6850.casb16010156

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

杂交水稻制种过程中籽粒内源激素含量的动态变化

李霞,宋一鸣,程凤娟,戴杏,王若仲   

  1. 湖南农业大学植物激素与生长发育湖南省重点实验室,湖南农业大学植物激素与生长发育湖南省重点实验室,湖南农业大学植物激素与生长发育湖南省重点实验室,湖南农业大学植物激素与生长发育湖南省重点实验室,湖南农业大学植物激素与生长发育湖南省重点实验室
  • 收稿日期:2016-01-31 修回日期:2016-05-10 接受日期:2016-03-22 出版日期:2016-06-01 发布日期:2016-06-01
  • 通讯作者: 王若仲
  • 基金资助:
    湖南省科技计划项目“水稻PLA3 介导miRNA靶基因翻译过程及其对种子萌发的调控机制”(2015JC3103)；公益性农业行业专项“主要农作物高活力种子生产关键技术与示范”(201303002)；湖南省自然科学基金“两系核不育系‘株1S’穗发芽调控研究”(2016JJ5039)；湖南省高校创新平台开放基金“水稻种子萌发中PLA3 基因表达及其与ABL1 的关系”(15K057)。

Changes of Endogenous Hormones Contents During Hybrid Rice Seed Production

  • Received:2016-01-31 Revised:2016-05-10 Accepted:2016-03-22 Online:2016-06-01 Published:2016-06-01

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摘要： 穗发芽对粮食生产造成巨大的经济损失，为研究穗发芽前杂交水稻制种中杂交种子籽粒内源激素含量变化关系。采用HPLC等方法，以2个杂交水稻组合‘Y两优1号’（亲本为Y58S× R9311×）和‘II优416’（ Ⅱ-32A×R416）为材料，在水稻制种过程中进行穗发芽实验。发现组合‘Y两优1’号种子比‘II优416’易于穗发芽。同时测定了两组合在授粉15 ~27 天期间种子内源激素（IAA﹑GA1﹑ZR和ABA）含量的变化，易穗发芽的‘Y两优1号’内源激素ZR﹑IAA和GA1含量高于‘II优416’，ABA含量低于‘II优416’，且‘Y两优1号’籽粒内源GA1/ABA比值在整个过程中均大于‘II优416’。由此可见，杂交水稻制种过程中出现穗发芽现象的最主要原因是穗发芽前期种子内GA1的含量高于ABA且在发生穗发芽时GA1/ABA比值比较大。

关键词: 冬小麦, 冬小麦, 灌浆期, 连阴雨, 等级, 指标

Abstract: Pre-harvest sprouting causes huge economic losses to food production. The purpose of this study is to investigate the changes of phytohormone contents in hybrid rice during pre-harvest sprouting. HPLC methods were employed to analyze phytohormones content in this paper. Two different hybrid rice, including ‘Y Liangyou 1’ (hybrid parent is R9311×Y58S) and ‘II You 416’ (hybrid parents to R416×Ⅱ-32A), were used as the material. The results indicated that the seed germination rate of ‘Y Liangyou 1’ was much higher than ‘II You 416’. The endogenous hormone (IAA, GA1 ZR and ABA) content changes were investigated in pollination seed during15 d to 27 d. The results showed that the contents of endogenous hormone IAA and ZR, GA1 in ‘Y Liangyou 1’ is higher than that in ‘II You 416’, but ABA content is lesser than ‘II You 416’, and the ratio of GA1/ABA in ‘Y Liangyou 1’ Grain Endogenous is greater than ‘II You 416’. The content of phytohormones GA1, ABA and the ratio of GA1/ABA are most important reasons for pre-harvest sprouting during hybrid rice seed production process.