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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (4): 34-39.doi: 10.11924/j.issn.1000-6850.2014-2545

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

羊柴种子去荚、低温层积处理后萌发特性及HPLC法测定内源激素ABA、IAA含量变化

牛亚洁,袁金凤,陈红贤,董倩倩,范亚丽,汪晓峰,刘忠华   

  1. (北京林业大学生物科学与技术学院,北京 100083）
  • 收稿日期:2014-09-19 修回日期:2014-11-04 接受日期:2014-11-25 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 刘忠华
  • 基金资助:
    林业公益性行业科研专项经费项目“种子超干及包覆技术在沙区生态修复中的应用研究”(201104024)。

Germination Characteristic and Endogenous ABA, IAA Content Change with HPLC in the Seed of Hedysarum laeve Maxim During Stratifying, Shelling

Niu Yajie, Yuan Jinfeng, Chen Hongxian, Dong Qianqian, Fan Yali, Wang Xiaofeng, Liu Zhonghua   

  1. (College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083)
  • Received:2014-09-19 Revised:2014-11-04 Accepted:2014-11-25 Online:2015-03-20 Published:2015-03-20

摘要： 为了研究羊柴种子自身萌发力对其飞播过程的影响，笔者对羊柴种子去荚处理后含水量、吸水规律、萌发能力以及羊柴种子不同天数低温层积处理后萌发能力进行相关研究，同时对2种处理后内源激素ABA、IAA分别进行生物测定。结果表明：带荚种子含水量为9.16%，去荚种子为8.92%；两者吸水过程均分为急剧吸水期、稳定吸水期、饱和吸水期3个阶段，去荚种子的最大吸水速率为带荚种子的4.79倍，达到饱和期所用时间约为带荚种子的1/2；羊柴种子去荚处理后发芽率、发芽指数最高，分别为81.67%、33.27，为对照组的8.167、38.39倍，带荚种子低温层积处理60天后发芽率、发芽指数次之，为46.67%、4.19；CK组抑制物ABA含量最高，达7921.12 ng/mL，经过80天低温层积处理后ABA含量降至最低，为4590.85 ng/mL。综合去荚处理以及低温层积处理后的发芽率、发芽指数、ABA含量，IAA/ABA比值，最后确定羊柴种子的最适发芽条件为去荚处理以提高其吸水性能或对带荚种子进行60天，4℃的低温层积处理。

关键词: 种群动态, 种群动态

Abstract: In order to determine the internal factors inhibited seed germination of Hedysarum laeve Maxim, the moisture, absorbent law, seed germination characteristics, endogenous ABA and IAA content of shelled or pods under cold stratification were studied. The results showed that the moisture of seeds with pods was 9.16%, while the shelled was 8.92%; the soaking process all included sharp soaking, stable soaking and saturated soaking stages. The maximum water absorption rate of the shelled reached 0.7475 g/h, which was 4.79 times of the pods, and the shelled spent about half time reaching saturated soaking stages compared with the pods. The shelled germination rate and germination index could attain 81.67% and 33.27, which were 8.167 and 38.39 times contrasted with the control group, pods with 60-day cold stratification was following, which reached 46.67% and 4.19; the inhibitor ABA content of CK was relatively the highest, reaching 7921.12 ng/mL, ABA content after 80-day cold stratification could drop down to 4590.85 ng/mL. If the germination percentage, germination index, endogenous ABA content and IAA/ABA ratio were all considered, it is suggested that the optimum condition to improve seed germination characteristics for Hedysarum laeve Maxim should be shelled to improve their water absorption or the pods treated by cold stratification at 4℃, 60-day.