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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (12): 50-53.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

苎麻咖啡酰辅酶A甲基转移酶基因原核表达及其结构分析

陈建荣,郭清泉,张学文,陈金军   

  • 收稿日期:2008-08-15 修回日期:2008-10-07 出版日期:2008-12-05 发布日期:2008-12-05

High-level Expression Ramie Caffeoyl-CoA 3-O-methyltransferase Gene in E.coli

Chen Jianrong, Guo Qingquan, Zhang Xuewen, Chen Jinjun   

  • Received:2008-08-15 Revised:2008-10-07 Online:2008-12-05 Published:2008-12-05

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摘要： 采用大肠杆菌表达系统pQE，对苎麻木质素合成关键基因咖啡酰辅酶A-甲基转移酶（CCoAOMT） cDNA编码区进行了高效表达，获得了分子量为29.4 kD的苎麻CCoAOMT融合蛋白。利用SwissModel服务器模拟构建了该酶蛋白的空间结构。并利用InsightII软件包中的Docking模块构建了该酶蛋白与辅酶Sinapoyl、SAH和Ca2+相互作用的复合物的结合模型，并利用Discover模块对模拟的结构进行结构优化。可以推知所克隆的苎麻CCoAOMT cDNA编码烟咖啡酰辅酶A甲基转移酶，具有O-甲基转移酶蛋白典型的催化功能，参与苎麻木质素单体前体的甲基化反应。

关键词: 南阳黄牛, 南阳黄牛, 人培牛黄, 试验, 回归分析

Abstract: Ramie caffeoyl-CoA 3- O-methyltransferase was expressed by pQE High-level Expression system in E.coli. The molecular weight of Ramie Fusion caffeoyl-CoA3- O-methyltransferase is 29.4 kD. The structure of the enzyme has been constructed by SwissModel server. Multiplex structure of the enzyme ,Sinapoyl CoA , SAH and Ca2+ has been constructed by Docking module in software InsightII,and has been optimized by Discover module. It can be presume that Ramie caffeoyl-CoA 3- O-methyltransferase has catalyse function of O-methyltransferase, must been related to lignin monomer precursor methyltransfer.