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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (18): 148-152.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

离心对蒺藜苜蓿花药愈伤组织生长和分化的影响

谷文英 祈新梅 蒋晓云 魏臻武 高洪文   

  • 收稿日期:2011-03-30 修回日期:2011-05-30 出版日期:2011-07-25 发布日期:2011-07-25
  • 基金资助:

    牧草种质资源苗期抗逆性评价鉴定

Effect of Centrifugation on Callus Induction and Differentiation of Medicago truncatula Gaertn Anther

  • Received:2011-03-30 Revised:2011-05-30 Online:2011-07-25 Published:2011-07-25

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摘要：

以蒺藜苜蓿杂交F1代花药为材料，检测了离心力、离心时间对不同基因型花药愈伤组织诱导、生长及分化的影响。结果表明：离心能促进花药愈伤组织的形成；对A基因型花药的诱导作用更明显；不同离心力处理的花药愈伤组织诱导率差异极显著（P<0.01），其中1000×g，2 min的效果最好；离心对愈伤组织的生长无明显影响，但对抗氧化酶的活性强弱有影响；随离心力的增大，过氧化物酶（POD）的活性逐渐增强，其变化趋势与过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的相反。

关键词: 醮花法, 醮花法, 拟南芥, 生殖细胞, 禾本科

Abstract:

The effects of centrifugal force and centrifugation time on callus induction and growth of anthers of Medicago truncatula Gaertn F1 hybrids from different genotypes were detected in this study. The results showed that centrifugation could promote the formation of anther callus, especially for the genotype A anthers. The callus induction rates of anthers were significantly different (P<0.01) after given different centrifugal force, the optimum pretreatment was 1000×g for 2min. Callus growth was not obviously affected by centrifugal force and centrifugation time. However, the strength of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) of callus were affected by centrifugal force. POD activity gradually increased with the increase of centrifugal force, and the trend of it was opposite with SOD and CAT.

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