新疆小拟南芥NPR1基因对植物系统获得性抗性的影响

doi:10.11924/j.issn.1000-6850.casb15120034

中国农学通报 ›› 2016, Vol. 32 ›› Issue (14): 44-49.doi: 10.11924/j.issn.1000-6850.casb15120034

所属专题：生物技术；农业生态

新疆小拟南芥NPR1基因对植物系统获得性抗性的影响

裴娟,欧秀玲,李凤,孔德真,王琨,易小龙,祝建波,王爱英

(石河子大学生命科学学院,石河子农业生物技术重点实验室,新疆石河子 832003）

收稿日期:2015-12-06 修回日期:2016-04-27 接受日期:2016-01-25 出版日期:2016-05-17 发布日期:2016-05-17
通讯作者: 王爱英
基金资助:
国家自然科学基金“小拟南芥NPR1基因第三内含子功能及其SAR分析”(C050103)。

Effect of NPR1 Gene from Arabidopsis pumila on Plant Systemic Acquired Resistance

Pei Juan, Ou Xiuling, Li Feng, Kong Dezhen, Wang Kun, Yi Xiaolong, Zhu Jianbo, Wang Aiying

(Shihezi Key Laboratory of Agriculture Biotechnology, College of Life Science, Shihezi University, Shihezi Xinjiang 832003)

Received:2015-12-06 Revised:2016-04-27 Accepted:2016-01-25 Online:2016-05-17 Published:2016-05-17

摘要/Abstract

摘要： 旨在研究小拟南芥NPR1基因的第三内含子对基因表达的影响。克隆出带有第三内含子的小拟南芥NPR1基因，构建诱导型植物表达载体并对拟南芥进行农杆菌遗传转化，经5 mmol/L外源水杨酸12 h诱导，利用Real-time PCR技术检测拟南芥病程相关蛋白的表达量。结果显示野生型拟南芥的NPR1、PR-1、PR-2、PR-5表达量高于转基因拟南芥；相对于野生型拟南芥，转基因拟南芥中SOD、POD酶活显著降低，而CAT酶活增加未达到显著水平。结果表明转入含有第三内含子的Ap.NPR1基因，转基因植株系统获得性抗性途径受到抑制，植株抗病性降低，说明第三内含子使得系统获得性抗性途径的基因表达受到抑制。

关键词: 硒, 硒, 富硒植物, 硒蛋白, 标准, 行业

Abstract: The aim is to study the effect of the third intron in Arabidopsis pumila’s NPR1 gene on gene expression. Arabidopsis pumila NPR1 gene was cloned with the third intron, plant expression vector of NPR1 gene was constructed and Arabidopsis thaliana were transformed via Agrobacterium tumefaciens. After being induced 12 h with 5 mmol/L exogenous salicylic acid, the expression of the pathogenesis-related protein was detected with Real-time PCR. The results showed that the expression of NPR1, PR-1, PR-2, PR-5 in wild-type Arabidopsis thaliana was higher than those of the transgenic Arabidopsis thaliana. Compared with wild-type Arabidopsis thaliana, the SOD and POD activities significantly decreased in transgenic Arabidopsis thaliana, while the CAT activity did not increase significantly. The results showed that when the gene Ap.NPR1 containing the third intron was transferred into Arabidopsis thaliana, the transgenic plants systemic acquired resistance pathway was inhibited, and the plant disease resistance was reduced, which indicated that the third intron inhibited the gene expression of the systemic acquired resistance pathway.

裴娟,欧秀玲,李凤,孔德真,王琨,易小龙,祝建波,王爱英. 新疆小拟南芥NPR1基因对植物系统获得性抗性的影响[J]. 中国农学通报, 2016, 32(14): 44-49.

Pei Juan,Ou Xiuling,Li Feng,Kong Dezhen,Wang Kun,Yi Xiaolong,Zhu Jianbo and Wang Aiying. Effect of NPR1 Gene from Arabidopsis pumila on Plant Systemic Acquired Resistance[J]. Chinese Agricultural Science Bulletin, 2016, 32(14): 44-49.

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新疆小拟南芥NPR1基因对植物系统获得性抗性的影响

Effect of NPR1 Gene from Arabidopsis pumila on Plant Systemic Acquired Resistance

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