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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (3): 38-40.doi: 10.11924/j.issn.1000-6850.2011-1572

所属专题： 生物技术； 水稻

• 农学 农业基础科学 • 上一篇    下一篇

水稻‘陆18S’核不育基因连锁的分子标记

李建军 肖层林   

  • 收稿日期:2011-05-24 修回日期:2011-10-17 出版日期:2012-01-25 发布日期:2012-01-25

Molecular study on male sterile link gene of Lu18S

  • Received:2011-05-24 Revised:2011-10-17 Online:2012-01-25 Published:2012-01-25

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摘要：

为了研究水稻不育基因的遗传机理和连锁情况，利用‘陆18S’与3个父本杂交，并对F2进行花粉育性和结实率观察和遗传分析，同时对F2后代进行RAPD分子标记。通过遗传分析得知，‘陆18S’不育系由1对隐性核基因控制。通过分子标记的筛选，找到了与‘陆18S’雄性不育紧密连锁的RAPD标记S490，片段大小为850 bp。获得与不育基因紧密连锁的RAPD标记。为不育基因克隆、定位奠定了坚实的基础。

关键词: 蚕丝业, 蚕丝业, 可持续发展, 发达地区, 基地建设, 建议

Abstract:

The aim was to study the linkage and genetic mechanisms of nuclear male sterile in rice. In this study, pollen fertility and seed setting observation were used to investigate in exploring crossing with three male varieties by bulk segregant analysis of F2 population between the parents and two bulks population representing fertile and sterile plants. The results indicated that male-sterile gene of ‘Lu18S’ belonged to one recessive nucleus gene inheritance by one RAPD primer S490 amplified a length of 850 bp fragments. It showed close linkage with sterile gene. The length of the fragment was coincided with Kotb’s result. The RAPD linkage markers were obtained. It provided the basis for the study of sterile gene clone and location.