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中国农学通报 ›› 2012, Vol. 28 ›› Issue (12): 192-197.doi: 10.11924/j.issn.1000-6850.2011-3430

所属专题: 生物技术

• 植物保护 农药 • 上一篇    下一篇

高粱靶斑病菌粗毒素对高粱胚根生长及胚根细胞膜透性的影响

张园园 徐秀德 王振东 张立军 姜钰 胡兰 董怀玉   

  • 收稿日期:2011-11-17 修回日期:2012-02-05 出版日期:2012-04-25 发布日期:2012-04-25
  • 基金资助:

    国家高粱产业技术体系项目

The Effect of Crude Toxins of Bipolaris sorghicola on Growth and Membrane Permeability of Sorghum Radicle

  • Received:2011-11-17 Revised:2012-02-05 Online:2012-04-25 Published:2012-04-25

摘要:

为了揭示高粱靶斑病菌的致病机理,用不同浓度病原菌粗毒素处理高粱种子L407B和Tx622B,测定其胚根生长抑制率、电导率和丙二醛(MDA)含量,探讨高粱靶斑病菌粗毒素对高粱不同品种胚根生长及胚根细胞膜透性的影响。结果表明,病菌粗毒素对高粱胚根生长有明显的抑制作用。在同一毒素浓度下,处理后2天,处理组胚根的相对电导率和MDA含量均高于对照组;同一处理时间内,不同浓度毒素处理后胚根相对电导率和MDA含量均高于对照组。说明高粱靶斑病菌毒素能降低高粱胚根细胞膜透性,且不同高粱品种间差异显著。

关键词: 环境管理, 环境管理, 场地修复, 风险评价

Abstract:

The aim was to clarify the pathogenic mechanism of Bipolaris sorghicola in sorghum. The seeds of L407B and Tx622B were treated by different concentrations of Bipolaris sorghicola crude toxins. And the radical growth inhibition rate as well as the electrical conductivity and malondialdehyde (MDA) content were detected to study the effect of crude toxins of Bipolaris sorghicola on the growth and membrane permeability of different varieties of sorghum radicle. The results showed that crude toxin could obviously inhibit growth of sorghum radical. The electrical conductivity and MDA content were all higher in the group which was treated by Bipolaris sorghicola crude toxins with same concentration two days before than in control group. The electrical conductivity and MDA content were also higher in the group which was treated by Bipolaris sorghicola crude toxins with different concentration on the same treated time. It demonstrated that Bipolaris sorghicola crude toxins could reduce membrane permeability of sorghum radicle and there was significant difference among the different varieties of sorghum.

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