4种熏蒸剂对土壤微生物特性的影响

doi:10.11924/j.issn.1000-6850.2012-0335

摘要/Abstract

摘要： 为了研究1,3-二氯丙烯、二甲基二硫、威百亩、甲酸甲酯4种土壤熏蒸剂处理7天对土壤微生物特性的影响。通过室内培养试验，采用稀释平板法测定土壤微生物细菌、放线菌和真菌的数量变化；熏蒸提取-茚三酮法测定土壤微生物生物量氮；直接吸收法测定土壤基底呼吸作用及基底诱导呼吸作用；高锰酸钾滴定法测定土壤过氧化氢酶活性及紫外分光光度法测定土壤中脱氢酶和淀粉酶活性。结果表明：按照熏蒸剂与土壤质量比为25 mg/kg的浓度胁迫条件下，熏蒸结束7天后4种熏蒸剂均显著减少土壤中细菌和放线菌的数量以及微生物生物量氮，但对真菌影响效果不大；1,3-二氯丙烯和威百亩能显著抑制土壤基底呼吸作用；威百亩对土壤酶活的影响效果显著。由此得出4种熏蒸剂均会对土壤微生物特性产生一定影响，尤以威百亩效果最显著，其次为1,3-二氯丙烯，而甲酸甲酯和二甲基二硫在影响微生物数量及微生物生物量氮方面效果显著。

关键词: 定殖, 定殖

Abstract: In order to research the effects of 4 kinds of soil fumigation such as the 1,3-dichloropropylene, dimethyl disulfide, metham sodium and formic acid methyl ester on the soil microbes characteristics after fumigation a week, and the experiments determined the quantity changes of soil microbial bacteria, fungi and actinomyces with dilution plate method through the indoor culture experiments. Soil microbial biomass nitrogen was determined by fumigation extraction-ninhydrin method; soil respiration was known by absorbed directly method; catalase activity was determined by permanganate titration; the activity of dehydrogenase and amylase were measured by ultraviolet spectrophotometric method. The results showed that, after fumigation 7 days 4 kinds of soil fumigation all significantly reduced the number of soil bacteria and actinomyces and microbial biomass nitrogen, but it had less effects on fungi. Also 1,3-dichloropropylene and metham sodium could significantly inhibited soil respiration. Among this, the effects of metham sodium on soil enzyme activity were most significant. Thus, 4 kinds of soil fumigation all took some effects on soil microorganism and metham sodium had the most significant, the next was dimethyl disulfide, but 1,3-dichloropropylene and formic acid methyl had some significant on microbial populations and microbial biomass nitrogen.

张腾燕平梅李园郭美霞曹坳程. 4种熏蒸剂对土壤微生物特性的影响[J]. 中国农学通报, 2013, 29(3): 116-120.

参考文献

[1] Stromberger M E, Klose S, Ajwa H A, et al. Microbial populations and enzyme activities in soils fumigated with methyl bromide alternatives[J].Soil Science Society of America Journal,2005,69(6): 1987-1999.
[2] Tanaka S, Kobayashi T, Iwasaki K, et al. Properties and metabolic diversity of microbial communities in soils treated with steam sterilization compared with methyl bromide and chloropicrin fumigations[J].Soil Science and Plant Nutrition,2003(49):603-610.
[3] Roux-michollet D, Czarnes S, Adam B, et al. Effects of steam disinfestation on community structure, abundance and activity of heterotrophic, denitrifying and nitrifying bacteria in an organic farming soil[J].Soil Biology and Biochemistry,2008,40(7): 1836-1845.
[4] Bending G D, Lincoln S D. Inhibition of soil nitrifying bacteria communities and their activities by glucosinolate hydroly-sis products[J].Soil Biology and Biochemistry,2000(32):1261-1269.
[5] Ibekwe M A. Effects of fumigants on non-target organisms in soils [J].Advances in Agronomy,2004(83):1-35.
[6] 曹志平.土壤生态学[M].北京:化学工业出版社,2007:41.
[7] 陈恩凤.土壤酶与土壤肥力研究[M].北京:科学出版社,1979:54-61.
[8] 崔俊涛.微生物在土壤腐殖质形成与转化中作用的研究[D].长春:吉林农业大学,2006:4.
[9] 程新胜,杨建卿.熏蒸处理对土壤微生物及硝化作用的影响[J].中国生态农业学报,2007,15(6):51-53.
[10] 陈云峰,曹志平,于永莉.甲基溴替代技术对番茄室温土壤养分及微生物量碳的影响[J].中国生态农业学报,2007,15(5):42-45.
[11] 张琦,裘炯良,尤明传,等.硫酰氟熏蒸灭鼠灭蠊效果及剂量与时间关系研究[J].中国媒介生物学及控制杂志,2011,22(5):443-445.
[12] 李南.土壤消毒的基本方法[J].农业科技与信息,2004,14(2):32-34.
[13] 计光辉,郑洪元.土壤微生物分析方法手册[M].北京:农业出版社, 1986:5-30.
[14] 沈萍,陈向东.微生物学实验[M].北京:高等教育出版社,2007,11(4): 16-19.
[15] 吴金水,林启美,黄巧云,等.土壤微生物生物量测定方法及其应用[M].北京:气象出版社,2006:6-72.
[16] 王占华,周兵,袁星.4种常见农药对土壤微生物呼吸的影响及其危害性评价[J].农药科学与管理.2005,26(6):13-15.
[17] 李振高,骆永明,滕应.土壤与环境微生物研究方法[M].北京:科学出版社,2008:345-346.
[18] 叶央芳,闵航,周湘池.苯噻草胺对水田土壤呼吸强度和酶活性的影响[J].土壤学报,2004,41(1)93-96.
[19] 乔雄梧,王静,秦曙,等.4种农药对土壤微生物的影响Ⅱ:氮素矿质化的变化[J].应用与环境生物学报,1999,5(l):158-161.
[20] 程新胜,杨建卿.熏蒸处理对土壤微生物及硝化作用的影响[J].中国生态报,2007,15(6):51-53.
[21] 孙军德,赵春燕,曲宝成,等.氯化苦熏蒸土壤对微生物种群数量的影响[J].土壤通报,2005,36(2):283-285.
[22] Bendavid-Val R, Rabinowitch H D, Katan J, et al. Viability of VA-mycorrhizal fungi following soil solarization and fumigation[J]. Plant and Soil,1997,195(1):185-193.
[23] 范昆,王开运,王东,等.1,3-二氯丙烯药剂对土壤微生物数量和酶活性的影响[J].生态报,2008,28(2):695-700.
[24] 傅丽君.4种农药对土壤微生物呼吸及过氧化氢酶活性的影响[J].福建农林大学学报,2005,34(4):441-445.