木本植物原生质体培养体系研究进展

doi:10.11924/j.issn.1000-6850.2012-2947

摘要/Abstract

摘要： 为了给木本植物原生质体培养技术体系的建立提供理论依据，推动木本植物快速繁殖和品种遗传改良工作的开展，从原生质体分离、纯化、培养方式等方面对木本植物原生质体培养体系进行了详细阐述，对比分析了原生质体不同分离方法和培养方式的优缺点，研究总结出影响原生质体培养的主要因素，并提出了木本植物原生质体培养今后的研究重点。

关键词: 原核表达, 原核表达

Abstract: In order to provide theoretical basis for establishment of protoplast culture system and promote the work of rapid propagation and genetic improvement of woody plants, a detailed description about protoplast culture system in woody plants from three aspects such as separation, purification, culture method and medium composition was made in the article. Advantages and disadvantages in different types of separation and culture method of protoplast were analyzed. The main influence factors on protoplast culture in woody plants were summerized. Significance for distant hybridization, new variety creation and genetic improvement by the way of protoplast culture in woody plants was brought out. Research emphasis on protoplast culture in woody plants was also proposed.

彭邵锋陆佳陈永忠王瑞陈隆升马力王湘南. 木本植物原生质体培养体系研究进展[J]. 中国农学通报, 2013, 29(1): 1-6.

PENG Shao-Feng chenyongzhong CHEN marry. Research Progress of Protoplast Culture in Woody Plants[J]. Chinese Agricultural Science Bulletin, 2013, 29(1): 1-6.

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