鸭疫里默氏菌噬菌体裂解酶的原核表达

doi:10.11924/j.issn.1000-6850.casb15070112

中国农学通报 ›› 2016, Vol. 32 ›› Issue (2): 5-8.doi: 10.11924/j.issn.1000-6850.casb15070112

所属专题：生物技术；畜牧兽医

鸭疫里默氏菌噬菌体裂解酶的原核表达

程龙飞,傅秋玲,陈红梅,傅光华,施少华,万春和,黄瑜

(福建省农业科学院畜牧兽医研究所,福州 350013）

收稿日期:2015-07-21 修回日期:2015-12-23 接受日期:2015-10-23 出版日期:2016-01-28 发布日期:2016-01-28
通讯作者: 黄瑜
基金资助:
现代农业产业技术体系建设专项“鸭病诊断与防治岗位”(CARS-43)。

Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer

Cheng Longfei, Fu Qiuling, Chen Hongmei, Fu Guanghua, Shi Shaohua, Wan Chunhe, Huang Yu

(Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013)

Received:2015-07-21 Revised:2015-12-23 Accepted:2015-10-23 Online:2016-01-28 Published:2016-01-28

摘要/Abstract

摘要： 为了原核表达鸭疫里默氏菌噬菌体RAP44的裂解酶基因，以噬菌体RAP44的基因组为模板，用PCR方法扩增裂解酶基因，与表达载体pGEX-6P-1连接，将重组质粒转化至大肠杆菌BL21(DE3)中表达，利用谷胱甘肽-Sepharose 4B亲和柱纯化表达蛋白。结果成功构建了表达载体pGEX-N，转化菌经IPTG诱导后成功表达出了分子量约为37 kD的可溶性重组蛋白。本研究获得了纯化的鸭疫里默氏菌噬菌体裂解酶重组蛋白，为裂解酶的功能研究奠定了基础。

关键词: 德国鸢尾, 德国鸢尾, 分株繁殖, 植物激素, 萌芽率

Abstract: The objective of this test was to make a prokaryotic express of the bacteriophage RAP44 lysin gene of Riemerella anatipestifer. The gene of lyase was amplified by PCR using genomic DNA of bacteriophage RAP44 and inserted into pGEX-6P-1 vector. The constructed recombinant plasmid was transformed into Escherichia coli BL21(DE3) to express fusion protein, which was purified by Glutathione-Sepharose 4B. The results showed that expression plasmid pGEX-N was constructed successfully. The soluble recombinant protein about 37 kD was expressed after induced by IPTG. The purified fusion protein was generated in this study, which laid a foundation for the functional study of bacteriophage lysin of Riemerella anatipestifer.

中图分类号:

S855.1

程龙飞,傅秋玲,陈红梅,傅光华,施少华,万春和,黄瑜. 鸭疫里默氏菌噬菌体裂解酶的原核表达[J]. 中国农学通报, 2016, 32(2): 5-8.

Cheng Longfei,Fu Qiuling,Chen Hongmei,Fu Guanghua,Shi Shaohua,Wan Chunhe and Huang Yu. Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer[J]. Chinese Agricultural Science Bulletin, 2016, 32(2): 5-8.

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鸭疫里默氏菌噬菌体裂解酶的原核表达

Prokaryotic Expression of Bacteriophage Lysin of Riemerella anatipestifer

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