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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (18): 206-210.doi: 10.11924/j.issn.1000-6850.2012-3081

• 食品 营养 检测 安全 • 上一篇    下一篇

大叶南五味子多糖纯化工艺优化

蒋红 王宏军 李新国 周铁忠   

  • 收稿日期:2012-09-10 修回日期:2012-10-12 出版日期:2013-06-25 发布日期:2013-06-25
  • 基金资助:
    KPS调控鸡免疫缺陷活性成分的结构解析及分子机制”;辽宁省重大动物疫病病原变异与综合防控技术研究

Purification technology of Kadsura polysaccharide from Kadsura marmorata fruits

  • Received:2012-09-10 Revised:2012-10-12 Online:2013-06-25 Published:2013-06-25

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摘要： 优化大叶南五味子干燥果实粗多糖(KPS)的纯化工艺。用三氯醋酸(TCA)法、Sevage法脱蛋白，单因素和正交试验法优化活性炭脱色工艺参数，比色法测定蛋白含量和脱色率。TCA法脱蛋白率达到72.6%，极显著高于Sevage法(P<0.01)；TCA法多糖损失率显著低于Sevage法(P<0.05)；活性炭脱色的最佳工艺参数：脱色温度60℃，脱色时间80 min，溶液pH 1，活性炭用量1.5%，脱色率达到86.83%±3.93%(n=6)，多糖含量达到19.39%±7.21% (n=6)。纯化KPS粗品最优工艺是用TCA法除蛋白质，1.5%的活性炭脱色。

关键词: 控制, 控制

Abstract: In order to purify crude kadsura polysaccharides(KPS) which were extracted from dried Kadsura marmorata fruits. The crude KPS were removed protein by trichloroacetic acid (TCA) method and Sevage method, respectively. On the base of single factor tests (decoloring temperature, decoloring time, solvent pH value and active carbon concentration), the conditions of active carbon decoloring method were optimized by the orthogonal test. The results showed that TCA method was the most appropriate removal protein method from the crude KPS and the ratio of removing protein reached to 72.6%. The optimal technological conditions of active carbon decoloring method was 60℃ of decoloring temperature, 80min of decoloring time, 1.0 of solvent pH value and 1.5% of active carbon concentration. In this condition, decoloring ratio reached to 86.83%. So the methods were simple, easy to apply, and suitable for the protein and pigment removal in crude KPS from K. marmorata fruits．