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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (13): 128-132.doi: 10.11924/j.issn.1000-6850.2012-3412

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

石榴果实花青苷差异性分析

刘安成 王亮生 王庆 庞长民   

  • 收稿日期:2012-10-18 修回日期:2012-11-15 出版日期:2013-05-05 发布日期:2013-05-05
  • 基金资助:
    陕西省科技计划经费资助项目

Analysis of Differences for Anthocyanins in Fruit of Pomegranate

  • Received:2012-10-18 Revised:2012-11-15 Online:2013-05-05 Published:2013-05-05

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摘要： 为了探讨石榴果实中花青苷的种类及果皮和果肉间的差异，采用高效液相色谱-光电二极管阵列检测器联用技术(HPLC-PAD)和高效液相色谱-电喷雾离子化-多级质谱联用技术(HPLC-ESI-MSn)，进行花青苷的定性及定量分析。结果发现，从11份不同产地观赏或食用石榴的果皮和果肉中，在525 nm共检测出10种花青苷成分，其中飞燕草素3-戊糖苷(Dp-3-pentoside)和芍药花素3-己糖苷(Pn-3-hexoside)首次从石榴果实中检测到。果皮和果肉中总花青苷含量无相关性，且种类也不完全相同。从检测的几种材料中发现，观赏石榴果皮中花青苷含量高于果肉，而食用品种果肉中的含量高于果皮。

关键词: 基因工程, 基因工程

Abstract: In order to find types and difference of anthocyanins in the peel and pulp of pomegranate, a high performance liquid chromatography with a photodiode array detector (HPLC-PAD) and HPLC electrospray ionization multiple mass spectrometry (HPLC-ESI-MSn) methods were employed for qualitative and quantitative analysis of anthocyanins. The results showed that: 10 anthocyanins were detected at 525 nm from 11 different origins of the ornamental or edible pomegranate peel and pulp, Delphinidin 3-pentoside and Peonidin -3-hexoside were first detected from pomegranate. It was not relativity in contents of anthocyanins in peel and pulp, and anthocyanins were not the same. The inspection found in several kinds of materials, anthocyanins contents in pulp of ornamental pomegranate were higher than in peel, and in peel of edible varieties were higher than in pulp.