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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (29): 37-41.doi: 10.11924/j.issn.1000-6850.2012-3690

所属专题： 生物技术； 畜牧兽医； 农业气象

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

炎热气候条件下母猪妊娠后期不同组织中GRα mRNA的表达规律研究

田启宇 陈永辉 范志勇 李铁军 刘仲华 邓近平   

  • 收稿日期:2012-11-13 修回日期:2012-12-13 出版日期:2013-10-15 发布日期:2013-10-15
  • 基金资助:
    国家自然科学基金;湖南省战略新兴产业科技攻关项目;湖南农业大学人才引进项目;湖南省教育厅重点项目;湖南省大学生创新性实验计划

Determination of GRα mRNA Expression in Tissues of Gilts during Late Gestation in Hot Weather

  • Received:2012-11-13 Revised:2012-12-13 Online:2013-10-15 Published:2013-10-15

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摘要： 本研究主要揭示炎热气候条件下母猪妊娠后期各组织器官GRα mRNA的表达规律，以期为GRα在母猪的热应激环境下的分子生理机制研究提供参考。试验选用12头妊娠母猪，根据妊娠日龄不同分为2组，每组6头，分别是妊娠90天和妊娠110天。按照常规方法屠宰母猪，迅速采集脑、卵巢、心脏、肝脏、脾脏、肺脏和肾脏，液氮速冻后-80°C保存，提取RNA，反转录，利用实时荧光定量PCR(qRT-PCR)方法检测各组织中GRα mRNA的相对表达量。结果显示，妊娠90天和110天时，GRα的mRNA表达丰度依次分别表现为：90天丰度，肺脏 > 脾脏 > 肝脏 > 肾脏 > 脑 > 卵巢 > 心脏；110天丰度，脾脏 > 肺脏 > 肝脏 >肾脏 > 卵巢 > 心脏 > 脑。其次，妊娠110天与90天相比，GRα mRNA的相对表达量除了在肺脏和脑中极显著降低(P<0.01)，在肝脏中显著降低之外(P<0.05)，其它组织则未表现出显著性变化(P>0.05)。结果表明，炎热气候条件下，母猪体内GRα mRNA优先在肺脏和脾脏中表达，其次是肝脏和肾脏，在脑、卵巢和心脏中的表达量最低，这种变化随妊娠时间延长更为明显，但同时表现出较明显的组织特异性。

关键词: 降解特性, 降解特性

Abstract: The study was conducted to reveal the GRα mRNA expression in different tissues of gilts during late gestation in high ambient temperature. The object of this study was to investigate the relationship between the GRα mRNA expression and physiological development of gilts during late gestation, to provide the basic reference data for the study of molecular mechanisms of heat stress. Twelve gilts (Landrace × Large White) during late gestation were assigned into two treatments (6 gilts/ treatment) according to the gestational day (90d and 110d). The gilts were slaughtered and tissues including brain, ovary, heart, liver, spleen, lung and kidney were collected aseptically over liquid nitrogen and frozen at -80°C. Then total RNA isolation and reverse transcription, quantitative real-time PCR(qRT-PCR) was performed to determine the mRNA expression of GRα in different tissues of gilts during late gestation. The results showed that the mRNA expression level of GRα in different tissues of gilts at d 90 of gestation was as follows: lung > spleen > liver > kidney > brain > ovary > heart; d 110: spleen > lung > liver > kidney > ovary > heart > brain. Compared d 110 with d 90 of gestation, except that the expression of GRα in lung and brain was significantly reduced (P<0.01), and was reduced (P<0.05) in liver, there were no gestation stage dependent effects on GRα mRNA expression in all the other tissues (P>0.05). Our results indicated that the mRNA expression of GRα was higher in lung and spleen of gilts during late gestation, to a lesser degree in liver and kidney, lower expression levels of GRα were observed in brain, ovary and heart. Compared d 110 with d 90 of gestation, the expression of GRα mRNA were reduced in lung, brain and liver at different degrees. this changes with prolonged pregnancy is more obvious, but at the same time showed a distinct tissue specificity.