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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (29): 32-36.doi: 10.11924/j.issn.1000-6850.2013-0089

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

五种中药有效成分诱导大鼠肠黏膜微血管内皮细胞分泌IFN-γ的研究

尹龙 李玉妹 孙静 张涛 段慧琴 董虹 穆祥 胡格   

  • 收稿日期:2013-01-09 修回日期:2013-04-09 出版日期:2013-10-15 发布日期:2013-10-15
  • 基金资助:
    IFN-γ在微血管内皮细胞免疫功能中的作用及机理研究;中药方剂防治动物传染性疾病机理及有效成分研究;微血管内皮细胞抗病毒活性与表达干扰素的关系研究;中药诱导气管上皮细胞表达抗病毒蛋白的研究;北京市科技新星计划项目

Induction of IFN-γ Production by Rat Intestinal Mucosal Microvascular Endothelial Cells bySome Active Ingredients of Traditional Chinese Medicines

  • Received:2013-01-09 Revised:2013-04-09 Online:2013-10-15 Published:2013-10-15

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摘要： 为了探讨抗病毒中药有效成分能否诱导肠黏膜微血管内皮细胞(IMMVECs)表达IFN-γ，本实验采用双夹心ELISA方法检测了穿心莲内酯、人参皂苷Rb1、苦参碱、绿原酸和甘草次酸等5种中药有效成分对体外培养的大鼠IMMVECs表达IFN-γ的影响。结果表明：一定浓度的穿心莲内酯、人参皂苷Rb1、苦参碱、绿原酸和甘草次酸均可以诱导大鼠IMMVECs表达IFN-γ，与空白对照组相比都具有极显著意义(P<0.01)。在同一个时间段内， 5.0 mg/L穿心莲内酯组IFN-γ的表达量高于10.0 mg/L穿心莲内酯组，20.0 mg/L人参皂苷Rb1组IFN-γ的表达量高于40.0 mg/L人参皂苷Rb1组，20.0 mg/L苦参碱组IFN-γ表达量高于40.0 mg/L 苦参碱组，5.0 mg/L 绿原酸组IFN-γ的表达量高于10.0 mg/L绿原酸组，5.0 mg/L甘草次酸组IFN-γ的表达量均高于10.0 mg/L甘草次酸。本结果提示：穿心莲内酯、人参皂苷Rb1、苦参碱、绿原酸和甘草次酸均可以激活MVECs诱生IFN-γ，而IFN-γ的表达可能与中药的抗病毒作用有直接或间接的关系。

关键词: 药敏试验, 药敏试验

Abstract: To determine whether the active ingredients of antiviral TCM induce the production of IFN-γ by cultured MVECs in vitro, the effects of andrographolide, ginsenoside Rb1, matrine, chlorogenic acid and glycyrrhetinic acid on it were assayed by ELISA method. The results showed that these five components all played significantly effect to IFN-γ production (P<0.01). Andrographolide, ginsenoside Rb1matrine chlorogenic acid and glycyrrhetinic acid at 5.0 mg/L, 20.0 mg/L，20.0 mg/L, 5.0 mg/L and 5.0 mg/L were higher than10.0 mg/L, 20.0 mg/L,40.0 mg/L, 10.0 mg/L and 10.0 mg/L，respectively. And 10.0 mg/L chlorogenic acid or glycyrrhetinic acid have lower roles than at 5.0 mg/L. The results suggested that above five components all could induced the secretion of IFN-γ in cultured IMMVECs and further play their antiviral functions which provide experimental basis for explaining the antiviral mechanism of TCM.