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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (32): 43-47.doi: 10.11924/j.issn.1000-6850.2014-0593

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

市售草鱼鳃中细菌的分离鉴定及耐药性分析

吕爱军   

  1. 江苏师范大学生命科学学院
  • 收稿日期:2014-03-07 修回日期:2014-03-07 接受日期:2014-09-17 出版日期:2014-11-20 发布日期:2014-11-20
  • 通讯作者: 吕爱军
  • 基金资助:
    国家自然科学基金项目“嗜水气单胞菌诱导斑马鱼皮肤免疫应答的分子机制”(30800847)，江苏省“青蓝工程”(2014)。

Identification and Antimicrobial Resistance Analysis of Bacteria Isolated from the Gills of Commercial Grass Carp (Ctenopharyngodon idellus)

  • Received:2014-03-07 Revised:2014-03-07 Accepted:2014-09-17 Online:2014-11-20 Published:2014-11-20

摘要： 对徐州市售草鱼细菌带染状况及耐药性分析，结果表明从其鳃中分离到4 株革兰氏阴性短杆菌，暂时编号为ZYF-1、ZYF-2、ZYF-3 和ZYF-4，查阅数值编码鉴定ZYF-1 菌株为志贺（Shigella sp.），ZYF-2、ZYF-3 菌株为大肠埃希菌（Escherichia coli），ZYF-4 菌株为泉居沙雷菌（Serratia fonticola）。药敏试验结果表明，4 株分离菌的耐药率分别为75.8%、72.4%、65.5%和82.8%，均对氨苄西林、阿莫西林、复方新诺明、磺胺异恶唑和甲氧苄啶等11 种抗生素耐药。本研究显示市售草鱼存在志贺菌、大肠埃希菌带染现象，对多种抗生素耐药，这为食源性细菌鉴定及鱼病防治提供参考。

关键词: 产量, 产量

Abstract: Investigation was conducted on bacterial contamination and antimicrobial resistance of the strains isolated from the gills of commercial Grass carp (Ctenopharyngodon idellus ) in Xuzhou, four isolated strains were gram-negative and short rod-shaped bacteria, and tentatively named ZYF-1, ZYF-2, ZYF-3 and ZYF- 4, respectively. According to the values of coding method of four strains, ZYF-1 was identified as Shigella sp., ZYF-2 and ZYF-3 were identified as Escherichia coli, and ZYF-4 was identified as Serratia fonticola. The antimicrobial susceptibility assay indicated that the resistance rates of ZYF-1, ZYF-2, ZYF-3 and ZYF-4 strains were 75.8%、72.4%、65.5% and 82.8%, respectively. Four strains showed antibiotic resistance to eleven kinds of antibiotics, such as ampicillin, amoxicillin, trimethoprim- sulfamethoxazole, sulfisoxazole, trimethoprim and so on. It is concluded that there are Shigella and Escherichia coli contamination in commercially availableCtenopharyngodon idellus in Xuzhou, and the bacteria showed multi-drug resistances in antimicrobial susceptibility assay. The conclusion will provide scientific references for identification of foodborne bacteria and control of bacterial disease in fish.