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中国农学通报 ›› 2013, Vol. 29 ›› Issue (19): 89-92.doi: 10.11924/j.issn.1000-6850.2012-3936

所属专题: 生物技术 烟草种植与生产

• 林学 园艺 园林 • 上一篇    下一篇

烟草N基因及其在烤烟遗传育种中的应用

张玉 罗成刚 杨爱国 常爱霞 冯全福   

  • 收稿日期:2012-12-05 修回日期:2013-01-04 出版日期:2013-07-05 发布日期:2013-07-05
  • 基金资助:
    中国烟草总公司科技重点项目;四川省烟草专卖局科技项目;中国农业科学院烟草研究所所长基金

Tobacco N Gene and Its Application in Genetic Breeding

  • Received:2012-12-05 Revised:2013-01-04 Online:2013-07-05 Published:2013-07-05

摘要: N基因起源于烟草野生种粘毛烟草(Nicotiana glutinosa),属于TIR-NBS-LRR类抗病基因,介导烟草花叶病毒(tobacco mosaic virus, TMV)的抗性,通过转座子标签法得到克隆,目前,N-TMV互作是研究最早且最多的植物-病原菌互作模型之一。文章从转录产物、表达特征、温度敏感性、对应无毒基因等研究内容回顾了N基因在结构、表达、作用机理等方面的研究进展及现状,归纳了以N基因为TMV抗源在烤烟遗传育种中的应用及取得的成果,并从抗TMV机制研究、抗TMV种质鉴定、种质资源利用等方面对N基因在烤烟抗TMV育种中的高效利用提出了展望。

关键词: 系统发育, 系统发育

Abstract: Abstract:The N gene, cloned from wild tobacco(Nicotiana glutinosa), is a type member of the Toll-IL-1 receptor homology region (TIR)-nucleotide binding site (NBS)-leucinerich repeat region (LRR) class of R genes and confers resistance to tobacco mosaic virus (TMV). The N gene was isolated by insertional mutagenesis using the Activator (Ac) transposon of maize,and N-TMV interaction is one of the earliest and most studied models of plant-pathogen interaction. In this article, the research progress of N gene in structure, expression, action mechanism were reviewed from the transcription products, expression characteristics, temperature sensitivity, corresponding avirulence gene and other aspects; The efficient using and achievements of N gene in TMV resistance breeding were summarized; And the prospect of efficient using of N gene was gave from three aspects, which is the mechanism research of resistant to TMV, identification and utilization of germplasm resources.