关键词: 水稻, 水稻, 钙, 光合特性, 亚细胞, 分布

Abstract: In order to breed herbicide resistant millets and master the molecular mechanism, the authors chose ACCase sequences from sethoxydim resistant line ‘Setaria italic Chum BC 6-1’ and sensitive line ‘S. italic Sda11’ to compare, and analyzed ACCase gene sequences of the 14 species. The base composition, transition and transversion, and genetic distance were calculated, and then the phylogenetic tree was constructed. The results showed that the lengths of ACCase sequence of the two lines had 2320 amino acids with 11 different amino acids. Except the reported I-1779-L locus showed a sethoxydim resistance, we found that the other 3 loci mutations, S-105-N, D-489-Y and W-876-L, might also show a sethoxydim resistance. The average 259 pairs of transition and 213 pairs of transversion appeared in 14 ACCase gene sequences. The phylogenetic trees were constructed by Neighbor Joining (NJ) method and Maximum Likelihood (ML) method, showing the same topologies. Phalaris paradoxa and Phalaris minor were first aggregated, subsequently, Avena sativa and Lolium rigidum. Another branch, Alopecurus myosuroides and Alopecurus japonicus were first aggregated, subsequently, Beckmannia syzigachne. Then the both were clustered. The third branch, Zea mays was aggregated, with Echinochloa crus-galli firstly, clustered with Setaria italic consequently. Only Triticum aestivum was separated, different from the traditional classification. This study found that the other 3 loci were related to sethoxydim resistance, which was contributed to understand the mechanism of herbicide resistance. It was the basis for molecular phylogenesis and herbicide resistant breeding.