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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (4): 155-159.doi: 10.11924/j.issn.1000-6850.casb15070174

所属专题： 资源与环境； 园艺

• 生物技术科学 • 上一篇    下一篇

新疆扁桃种质资源ITS序列分析及系统发育研究

朱 玲1,2,徐崇志1,2,张 锐1,2   

  1. (1塔里木大学植物科学学院,新疆阿拉尔 843300；2塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔 843300）
  • 收稿日期:2015-07-29 修回日期:2015-08-22 接受日期:2015-08-31 出版日期:2016-02-01 发布日期:2016-02-01
  • 通讯作者: 张 锐
  • 基金资助:
    新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题“扁桃F1代群体遗传连锁框架图的构建”(BRYB1007)。

Analysis of ITS Sequence and Research on Phylogeny of Amygdalus communis L. in Xinjiang

Zhu Ling1,2, Xu Chongzhi1,2, Zhang Rui1,2   

  1. (1College of Plant Sciences, Tarim University, Alar Xinjiang 843300;2Xinjiang Production and Construction Corps, Key Laboratory of Biological Resources Protection and Using in Tarim Basin, Alar Xinjiang 843300)
  • Received:2015-07-29 Revised:2015-08-22 Accepted:2015-08-31 Online:2016-02-01 Published:2016-02-01

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摘要： 本研究旨在为新疆扁桃种质资源的分子鉴定和遗传多样性研究提供依据。以24份扁桃种质为材料，提取DNA后对ITS序列进行PCR扩增及测序，测序结果与序列KC862380(Prunus lusitanica var.)比对，获得ITS1、5.8S与ITS2序列。利用相关生物信息学软件对ITS1与ITS2序列分别计算变异位点和简约信息位点等，构建UPGMA系统发育树。结果显示，ITS1与ITS2序列对位后长度分别为230 bp和277 bp，含丰富的变异位点和简约信息位点。基于ITS1序列的UPGMA树的遗传距离在0.00~0.01之间，‘鹰嘴’、‘矮丰’及‘石子’各自成一支。基于ITS2序列的UPGMA树其遗传距离在0.000~0.006之间，‘鹰嘴’与‘石子’聚为一支。研究表明，5.8S rDNA序列保守性极强，ITS1序列的变异信息大于ITS2序列，并且其碱基的缺失和替换多于ITS2序列，可能是导致系统发育树结果存在差异的原因。

关键词: 乡村旅游, 乡村旅游, 调研, 制约因素, 转型升级

Abstract: This experiment aimed to provide the basis for molecular identification and genetic diversity of Amygdalus communis L. germplasm resources in Xinjiang. This experiment used 24 germplasms of Amygdalus communis L. as the materials, PCR amplification and sequencing of the ITS sequence were carried out after extracting DNA. The sequencing results were compared with KC862380 (Prunus lusitanica var.), and then ITS1, 5.8S and ITS2 sequences were obtained. Then the authors used relevant bioinformatics software to calculate the mutation sites and simple information sites, etc. of ITS1 and ITS2 sequences. The UPGMA phylogenetic tree was built. The results showed that, after being aligned, the sequence length of ITS1 and ITS2 were 230 bp and 277 bp, respectively, and they had rich mutation sites and simple information sites. Based on ITS1, the genetic distance of UPGMA phylogenetic tree was 0.00-0.01, ‘Yingzui’, ‘Aifeng’ and ‘Shizi’ were divided into respective branch. Based on ITS2, the genetic distance of UPGMA phylogenetic tree was 0.000-0.006, ‘Yingzui’ and ‘Shizi’ were divided into the same branch. This test showed that 5.8S rDNA sequence had strong conservation. The variation information of ITS1 sequence was greater than that of ITS2 sequence, and the bases lack and replace phenomena of ITS1 sequence were more than those of ITS2 sequence, which might be the reason of phylogenetic tree differences.

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