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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (33): 346-350.doi: 10.11924/j.issn.1000-6850.2012-4194

所属专题： 油料作物

• 植物保护 农药 • 上一篇    下一篇

黑龙江向日葵菌核病拮抗菌株的分离与筛选

王春 王芊   

  • 收稿日期:2012-12-28 修回日期:2013-01-31 出版日期:2013-11-25 发布日期:2013-11-25

Isolation and Screening of Antagonistic Strains for Control of Sunflower Sclerotinic Rot

  • Received:2012-12-28 Revised:2013-01-31 Online:2013-11-25 Published:2013-11-25

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摘要： 为筛选出黑龙江向日葵菌核病的有效生防菌株，采集向日葵根围土、病株残体74份，采用土壤稀释平板法分离菌株和平板对峙培养初筛，共分离筛选出38株对向日葵菌核病有拮抗活性的生防菌株。通过菌核萌发抑制、离体叶片测试、温室盆栽试验复筛。结果表明，M1M2菌株表现最好，菌核萌发抑制效果和离体叶片防效均超过90%，其100 mL发酵原液处理土壤在温室盆栽中防效达83.52%，具备进一步研究应用的价值。

关键词: 图像处理, 图像处理

Abstract: For screening Antagonistic organism against Sclerotinia sclerotiorum efficiently, 74 soil samples of Heilongjiang Province were collected, isolated through dilution plate method and primarily screened through confronting incubation on PDA plate. The result showed that 38 antagonistic organism against Sclerotinia sclerotiorum were isolated and screened from 303 strains isolated from 74 soil samples. In the test of antagonistic strains on the germination of sclerotia and on detached leaves, M1M2 was found efficiently suppressing Sclerotinia sclerotiorum with more than efficacy of 90%. The efficacy of 83.52% was obtained in biocontrol test in the greenhouse by treating soil with 100ml fermented liquid. So M1M2 was a prospect strain with the value of further research and application.