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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (25): 184-189.doi: 10.11924/j.issn.1000-6850.2013-0082

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

猕猴桃根中三萜类化合物的提取工艺研究

田娜 陈颖韬 吴文杰 张盛 刘硕谦   

  • 收稿日期:2013-01-09 修回日期:2013-03-22 出版日期:2013-09-05 发布日期:2013-09-05
  • 基金资助:
    武陵山区特色资源高效综合利用关键技术研究与示范

Optimization of Extraction Process for Triterpenoids from the Roots of Radix Actinidiae Chinensis

  • Received:2013-01-09 Revised:2013-03-22 Online:2013-09-05 Published:2013-09-05

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摘要： 【研究目的】对猕猴桃根中的三萜类化合物的提取工艺进行研究，获得最佳的提取工艺条件；【方法】采用单因素实验考察提取温度、乙醇体积分数、提取时间、料液比与提取次数对三萜类化合物得率的影响，并以正交试验进一步优化提取工艺条件；【结果】猕猴桃根三萜类物质的最优提取工艺条件为提取温度80℃，乙醇体积分数65%，提取时间1.5 h，料液比1:20 (g/mL)，提取次数1次，在此工艺条件下，猕猴桃根三萜类物质的平均得率为6.7517%；【结论】该工艺应用于猕猴桃根中三萜类物质的提取简单可行，有较好的参考价值。

关键词: 联合毒力, 联合毒力

Abstract: 【Objective】The study was aimed to optimize the extraction of the triterpenoids from the roots of Radix Actinidiae Chinensis and obtain the optimal extraction process. 【Methods】 The effect of extraction temperature, ethanol concentration (volume percent), extraction time, material-to-liquid ratio and the number of extraction times on the yield of triterpenoids is investigated through single factor tests and orthogonal tests. 【Results】The optimal extraction conditions were determined as follows: extraction temperature of 80℃, ethano concentration of 65%, extraction time of 1.5 h, material-to-liquid ratio of 1:20 (g/mL) and the number of extraction times was one time. Under the optimized extraction conditions, 6.7517% of the extraction yield of triterpenoids from Radix Actinidiae Chinensis was achieved. 【Conclusion】This optimized extraction process is simple, feasible and has a very good reference value.