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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (22): 125-128.doi: 10.11924/j.issn.1000-6850.2013-0246

• 林学 园艺 园林 • 上一篇    下一篇

沙田柚果腐病病原菌的鉴定与生物学特性

董章勇 罗梅 宾淑英 肖志娜   

  • 收稿日期:2013-01-23 修回日期:2013-03-08 出版日期:2013-08-05 发布日期:2013-08-05
  • 基金资助:
    广东省自然科学基金;广东省大学生创新实验项目

Identification and Biological Characteristics of the Pathogen Causing Fruit Rot Disease of Pomelo

  • Received:2013-01-23 Revised:2013-03-08 Online:2013-08-05 Published:2013-08-05

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摘要： 沙田柚果腐病是近年为害沙田柚柚果的主要病害。根据病原菌的形态，结合分子鉴定的方法，将该病的致病菌鉴定为可可毛色二孢(Lasiodiplodia theobromae)。该病菌生长的最适温度为25~30℃；可生长的pH范围为4~11；该菌能有效利用蔗糖、葡萄糖、可溶性淀粉等7种供试碳源，以果糖最好；能利用甘氨酸、亮氨酸等8种供试氮源，以甘氨酸最适，无法利用尿素。

关键词: 种质资源, 种质资源

Abstract: Fruit Rot Disease was one of the most important disease harming pomelo in recent years. The pathogen was identified as Lasiodiplodia theobromae according to the morphology and sequence analysis of the internal transcribed spacer region of the ribosomal DNA (ITS). The optimum temperature for mycelial growth was 25-30℃. The pathogen could grow under pH 4-11. Fructose and glycine could be used as better carbon and nitrogen sources for growth of L.theobromae, respectively. Urea cannot be effectively used.